AB250998

Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal ENO1 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2, Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3

4 Images

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

This data was developed using ab189892, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-3 : 5 seconds; Lane 4 : 30 seconds.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (<a href='/en-us/products/primary-antibodies/eno1-eno2-eno3-antibody-epr18422-ab189892'>ab189892</a>) at 1/5000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Lane 4:

Human fetal spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Exposure time: 5s

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

This data was developed using ab189892, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (<a href='/en-us/products/primary-antibodies/eno1-eno2-eno3-antibody-epr18422-ab189892'>ab189892</a>) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Exposure time: 3s

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

This data was developed using ab189892, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human ENO1 full length recombinant protein (Cat no. : ab89248) containing aa1-434.

Human ENO2 full length recombinant protein containing aa1-434 with a His-Tag^®.

Human ENO3 full length recombinant protein (Cat no. : ab113127) containing aa1-434 with a His-Tag^®.

Human ENO2 full length recombinant protein was made in-house.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (<a href='/en-us/products/primary-antibodies/eno1-eno2-eno3-antibody-epr18422-ab189892'>ab189892</a>) at 1/5000 dilution

Lane 1:

Human ENO1 full length recombinant protein at 0.02 µg

Lane 2:

Human ENO2 full length recombinant protein at 0.02 µg

Lane 3:

Human ENO3 full length recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Exposure time: 5s

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

This data was developed using ab189892, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-4 and 8-10 : 1 second; Lanes 5-7 : 3 seconds.

Lanes 1 - 7:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (<a href='/en-us/products/primary-antibodies/eno1-eno2-eno3-antibody-epr18422-ab189892'>ab189892</a>) at 1/5000 dilution

Lanes 8 - 10:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (<a href='/en-us/products/primary-antibodies/eno1-eno2-eno3-antibody-epr18422-ab189892'>ab189892</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat spleen lysate at 10 µg

Lane 8:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 9:

Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 10:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Exposure time: 1s

Unconjugated

Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18422

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

ab250998 is the carrier-free version of ab189892.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENO1 ENO2 and ENO3 also known as alpha gamma and beta enolase are isoenzymes involved in the glycolytic pathway. Each enolase isoenzyme offers specific mechanical functions in this energy-producing process. ENO1 has an approximate mass of 47 kDa and is widely expressed in most tissues. ENO2 similar in size is primarily found in neural and neuroendocrine tissues. ENO3 also roughly 47 kDa predominantly expresses in muscle tissue. These proteins catalyze the reversible conversion of 2-phosphoglycerate to phosphoenolpyruvate playing an important role in glycolysis.

Biological function summary

The enolase family of isoenzymes acts as multifunctional catalysts and has roles beyond glycolysis. ENO1 serves as a plasminogen receptor on cell surfaces assisting in tissue remodeling and immune response. ENO2 often referred to as neuron-specific enolase (NSE) functions importantly in neural development and tumor cell metabolism. ENO3 or muscle-specific enolase contributes to muscle metabolism and energy turnover. While these proteins individually carry out essential tasks they also form a complex that facilitates efficient energy production and cellular regulation.

Pathways

The enolase enzymes integrate in the glycolytic and gluconeogenesis pathways central to energy metabolism. Glycolysis involves other key proteins like hexokinase and phosphofructokinase cooperating for glucose breakdown and energy release. In gluconeogenesis the enolases help generate glucose from non-carbohydrate sources important during fasting or intense exercise. Their activity along with related proteins impacts the efficiency and regulation of energy metabolism pathways.

Enolase dysregulation associates with conditions like cancer and neuromuscular diseases. Elevated ENO2 levels often serve as a biomarker for neuroendocrine tumors and a target for cancer therapies. ENO3 mutations link to muscle metabolic disorders including glycogen storage diseases. ENO1 interaction with proteins such as plasminogen can influence cancer cell invasion and metastasis highlighting its relevance in disease progression and potential therapeutic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 1369209, PubMed : 29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

See full target information ENO1

Additional targets

ENO2,ENO3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 14: PubMed37239471

2023

Differential Regulation of POC5 by ERα in Human Normal and Scoliotic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Hassan,Edward T Bagu,Shunmoogum A Patten,Sirinart Molidperee,Stefan Parent,Soraya Barchi,Isabelle Villemure,André Tremblay,Florina Moldovan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (AB250998)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise