AB222514

Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal ENO1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 2 publications.

View Alternative Names

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2, Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3

10 Images

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixedHeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ENO1 + ENO2 + ENO3with ab222514 at 1/800 (red) compared with an isotype control Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Secondary antibody Goat anti rabbit IgG Alexa Fluor^® 488 (ab150077), at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemical analysis of paraffin embedded human pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on medium-small ducts of human pancreas (PMID : 19425054). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemical analysis of paraffin embedded human clear cell carcinoma tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and nuclear staining on human clear cell kidney carcinoma is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunofluorescent analysis of 100% methanol fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.

Counterstains : ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor^® 594) (1/200). DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

Counterstains : ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor^® 594) (1/200). DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemical analysis of paraffin embedded mouse pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on the medium-small ducts of normal mouse pancreas is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lane 1-3 : 3 seconds

Lane 4 : 1 seconds

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 1:

Human fetal heart at 10 µg

Lane 2:

Human fetal kidney at 10 µg

Lane 3:

Human fetal spleen at 10 µg

Lane 4:

Human fetal brain at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lane 1/2/4 : 1 seconds

Lane 3 : 3 seconds

Lanes 1 - 3:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 4:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/10000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 3:

Human skeletal muscle at 10 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lanes 1 & 2 : 3 seconds

Lane 3 : 1 seconds

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/1000 dilution

Lane 1:

His-tagged human ENO1 (aa2-434) recombinant protein, 10 ng

Lane 2:

His-tagged human ENO2 (aa1-434) recombinant protein, 10 ng

Lane 3:

His-tagged human ENO3 (aa1-434) recombinant protein, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Blocking : 5% NFDM/TBST.

Exposure times :

Lane 1, 2 and 4 : 3 minutes

Lane 3 : 1 minute

Lane 5 : 3 seconds

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 1:

Mouse brain at 10 µg

Lane 2:

Mouse spleen at 10 µg

Lane 3:

Mouse kidney at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa

false

Carrier free

Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19680

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/800", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENO1 ENO2 and ENO3 also known as alpha gamma and beta enolase are isoenzymes involved in the glycolytic pathway. Each enolase isoenzyme offers specific mechanical functions in this energy-producing process. ENO1 has an approximate mass of 47 kDa and is widely expressed in most tissues. ENO2 similar in size is primarily found in neural and neuroendocrine tissues. ENO3 also roughly 47 kDa predominantly expresses in muscle tissue. These proteins catalyze the reversible conversion of 2-phosphoglycerate to phosphoenolpyruvate playing an important role in glycolysis.

Biological function summary

The enolase family of isoenzymes acts as multifunctional catalysts and has roles beyond glycolysis. ENO1 serves as a plasminogen receptor on cell surfaces assisting in tissue remodeling and immune response. ENO2 often referred to as neuron-specific enolase (NSE) functions importantly in neural development and tumor cell metabolism. ENO3 or muscle-specific enolase contributes to muscle metabolism and energy turnover. While these proteins individually carry out essential tasks they also form a complex that facilitates efficient energy production and cellular regulation.

Pathways

The enolase enzymes integrate in the glycolytic and gluconeogenesis pathways central to energy metabolism. Glycolysis involves other key proteins like hexokinase and phosphofructokinase cooperating for glucose breakdown and energy release. In gluconeogenesis the enolases help generate glucose from non-carbohydrate sources important during fasting or intense exercise. Their activity along with related proteins impacts the efficiency and regulation of energy metabolism pathways.

Enolase dysregulation associates with conditions like cancer and neuromuscular diseases. Elevated ENO2 levels often serve as a biomarker for neuroendocrine tumors and a target for cancer therapies. ENO3 mutations link to muscle metabolic disorders including glycogen storage diseases. ENO1 interaction with proteins such as plasminogen can influence cancer cell invasion and metastasis highlighting its relevance in disease progression and potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 1369209, PubMed : 29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

See full target information ENO1

Additional targets

ENO2,ENO3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1636367 PubMed40951359

2025

Claudin18.2 defines a prognostically distinct subgroup of intrahepatic cholangiocarcinoma via CD8 T-cell exclusion.

Applications

Unspecified application

Species

Unspecified reactive species

Chengui Yu,Yuren Pan,Fuli Li,Zhenyun Guo,Da Xu,Ying Zhu,Baobing Yin

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680]

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (AB222514)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise