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Rabbit Recombinant Monoclonal NSE antibody. Neuron marker. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 28 publications.


Images

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Rat
Tested
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use at 1/100 - 1/250.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

For unpurified use at 1/5000 - 1/20000.

Species
Rat
Dilution info
1/1000
Notes

For unpurified use at 1/5000 - 1/20000.

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/5000 - 1/20000.

Tested
Tested

Species
Human
Dilution info
1/20 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Additional Targets

ENO1, ENO3

Alternative names

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

Recommended products

Rabbit Recombinant Monoclonal NSE antibody. Neuron marker. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 28 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3377
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.

Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

Associated diseases and disorders

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757), expandable thumbnail

    Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757)

    ENO1 + ENO2 + ENO3 Western blot staining using rabbit Anti-ENO1 + ENO2 + ENO3 antibody

    All lanes: Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757) at 1/1000 dilution

    Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Y79 (Human retinoblastoma retinoblastoma) whole cell lysate at 20 µg

    Lane 4: Mouse brain lysate at 20 µg

    Lane 5: Rat brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757)

    ENO1 + ENO2 + ENO3 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-ENO1 + ENO2 + ENO3 antibody

    Immunocytochemistry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/50 dilution (2.0 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757)

    ENO1 + ENO2 + ENO3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ENO1 + ENO2 + ENO3 antibody

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757)

    ENO1 + ENO2 + ENO3 Flow Cytometry (Intracellular) staining using rabbit Anti-ENO1 + ENO2 + ENO3 antibody

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling NSE with purified ab79757 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757)

    ENO1 + ENO2 + ENO3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ENO1 + ENO2 + ENO3 antibody

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757)

    ENO1 + ENO2 + ENO3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-ENO1 + ENO2 + ENO3 antibody

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757)

    ENO1 + ENO2 + ENO3 Western blot staining using rabbit Anti-ENO1 + ENO2 + ENO3 antibody

    NSE western blot using anti-NSE antibody [EPR3377] - Neuronal Marker ab79757. Publication image and figure legend from Prieto-Fernández, E., Aransay, A. M., et al., 2019, Theranostics, PubMed 31367240.


    ab79757 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab79757 please see the product overview.

    Size-exclusion chromatography analysis of CSF. A, Western blot analysis of CD63, CD9, neuron-specific enolase (NSE), and human serum albumin (HSA) proteins. Molecular weights are shown in KDa. B, the number of TaqMan RT-qPCR replicates (from a total of 12) in which the studied miRNAs were detected. C, quantification (relative to ath-miR-159a) of miR-21-5p, miR-451a, and miR-92a-3p and D, of miR-1911-5p, miR-22-3p, and miR-30c-5p.

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