Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

Immunocytochemistry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/50 dilution (2.0 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling NSE with purified ab79757 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling ENO1 + ENO2 + ENO3 with purified ab79757 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Anti-DDDDK tag antibody [6F7] - Loading Control (ab205606) staining at 1/1000 dilution.

The cross-reactivity with ENO2 is weaker than that with ENO1 and ENO3

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757) at 1/1000 dilution

Lane 1:

Recombinant human ENO1 protein with C-DDDDK tag at 10 ng

Lane 2:

Recombinant human ENO2 protein with C-DDDDK tag at 10 ng

Lane 3:

Recombinant human ENO3 protein with C-DDDDK tag at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa

false

Exposure time: 180s

• WB

Unknown

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Y79 (Human retinoblastoma retinoblastoma) whole cell lysate at 20 µg

Lane 4:

Mouse brain lysate at 20 µg

Lane 5:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 47 kDa

false

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

Mouse anti-6x HisTag was used as a loading control.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (ab79757) at 1/1000 dilution

Lane 1:

Human ENO3 protein (His Tag) at 0.2 µg

Lane 2:

Human ENO2 protein (His Tag) at 0.2 µg

Lane 3:

Human ENO1 protein (His Tag) at 0.2 µg

Predicted band size: 47 kDa

false

• WB

CiteAb

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR3377] - Neuronal Marker (AB79757)

NSE western blot using anti-NSE antibody [EPR3377] - Neuronal Marker ab79757. Publication image and figure legend from Prieto-Fernández, E., Aransay, A. M., et al., 2019, Theranostics, PubMed 31367240.

ab79757 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab79757 please see the product overview.

Size-exclusion chromatography analysis of CSF. A, Western blot analysis of CD63, CD9, neuron-specific enolase (NSE), and human serum albumin (HSA) proteins. Molecular weights are shown in KDa. B, the number of TaqMan RT-qPCR replicates (from a total of 12) in which the studied miRNAs were detected. C, quantification (relative to ath-miR-159a) of miR-21-5p, miR-451a, and miR-92a-3p and D, of miR-1911-5p, miR-22-3p, and miR-30c-5p.

false