AB157474

Anti-ENO1 + ENO3 antibody [EPR11366(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal ENO3 antibody. Suitable for WB and reacts with Human, Recombinant fragment samples. Cited in 2 publications.

View Alternative Names

Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3

3 Images

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Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

All lanes:

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Lane 4:

Fetal heart lysate at 10 µg

Lane 5:

Fetal muscle lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

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Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Note : primary antibody diluted with 1% BSA.

All lanes:

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from fetal muscle lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG

Predicted band size: 47 kDa

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Lab

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Mouse anti-6x HisTag was used as a loading control.

All lanes:

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (ab157474) at 1/2000 dilution

Lane 1:

Human ENO3 protein (His Tag) at 0.2 µg

Lane 2:

Human ENO2 protein (His Tag) at 0.2 µg

Lane 3:

Human ENO1 protein (His Tag) at 0.2 µg

Predicted band size: 47 kDa

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Carrier free

Anti-ENO1 + ENO3 antibody [EPR11366(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11366(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENO3 protein also known as beta-enolase mechanically functions as an enzyme involved in glycolysis catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This protein has a molecular mass of approximately 47 kDa. It is specifically expressed in skeletal muscle tissue where it plays a critical role in energy metabolism. Beta-enolase is an isoform of enolase that is distinct from the alpha (ENO1) and gamma (ENO2) isoforms which have different tissue specificities.

Biological function summary

Beta-enolase supports cellular energy production in muscles. It does not typically form part of a protein complex but acts independently in the glycolytic pathway. Its function in glycolysis is important for providing ATP to muscle cells especially during periods of high energy demand such as exercise. The efficient conversion of metabolic substrates in this pathway is essential for muscle function and maintenance.

Pathways

Beta-enolase operates within the glycolytic pathway and plays an important role in energy production. This pathway is fundamental for breaking down glucose to provide ATP the energy currency of the cell. Beta-enolase works closely with other key glycolytic enzymes such as hexokinase and phosphofructokinase. Its role is indispensable in maintaining the flow of carbon through glycolysis ensuring robust energy supply for muscle contraction.

Beta-enolase is associated with diseases like glycogen storage disease type XIII and muscle-related disorders. Mutations in ENO3 gene can lead to reduced energetic efficiency and impact muscular health. Of particular note is the connection with related glycolytic proteins which may exhibit compensatory or contributing roles in these disorders. This highlights the importance of beta-enolase and its interactions in maintaining muscle physiology and energy homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.

See full target information ENO3

Additional targets

ENO1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Plant journal : for cell and molecular biology 120:123-138 PubMed39145515

2024

The eukaryotic translation initiation factor eIF4E regulates flowering and circadian rhythm in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Huanhuan Jin,Yadi Chen,Yonghong Jiang,Lianfeng Gu,Guifang Lin,Chentao Lin,Qin Wang

Mediators of inflammation 2023:8052579 PubMed37448887

2023

LINC00174 Promotes Colon Cancer Progression by Regulating Inflammation and Glycolysis by Targeting the MicroRNA-2467-3p/Enolase 3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Xu,Jiawei Lin,Rong Chen,Junjie Xie,Enquan Yuan,Fajie Cen,Fanbiao Kong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENO1 + ENO3 antibody [EPR11366(B)]

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

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Product promise