Rabbit Polyclonal ENO3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENO3.
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Publications
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- Frontiers in pharmacology 14:11279312023Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArenbaoligao,Xinrui Guo,Jiahao Xiong,Shuangshuang Zhang,Yuewen Yang,Dapeng Chen,Yu XiePubMed 37006994
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ENO3. Database link P13929
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Pig | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.5-5 µg/mL | Notes - |
Species Human | Dilution info 0.5-5 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.
Alternative names
Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3
Recommended products
Rabbit Polyclonal ENO3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENO3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ENO3. Database link P13929
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ENO3 protein also known as beta-enolase mechanically functions as an enzyme involved in glycolysis catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This protein has a molecular mass of approximately 47 kDa. It is specifically expressed in skeletal muscle tissue where it plays a critical role in energy metabolism. Beta-enolase is an isoform of enolase that is distinct from the alpha (ENO1) and gamma (ENO2) isoforms which have different tissue specificities.
- Biological function summary
Beta-enolase supports cellular energy production in muscles. It does not typically form part of a protein complex but acts independently in the glycolytic pathway. Its function in glycolysis is important for providing ATP to muscle cells especially during periods of high energy demand such as exercise. The efficient conversion of metabolic substrates in this pathway is essential for muscle function and maintenance.
- Pathways
Beta-enolase operates within the glycolytic pathway and plays an important role in energy production. This pathway is fundamental for breaking down glucose to provide ATP the energy currency of the cell. Beta-enolase works closely with other key glycolytic enzymes such as hexokinase and phosphofructokinase. Its role is indispensable in maintaining the flow of carbon through glycolysis ensuring robust energy supply for muscle contraction.
- Associated diseases and disorders
Beta-enolase is associated with diseases like glycogen storage disease type XIII and muscle-related disorders. Mutations in ENO3 gene can lead to reduced energetic efficiency and impact muscular health. Of particular note is the connection with related glycolytic proteins which may exhibit compensatory or contributing roles in these disorders. This highlights the importance of beta-enolase and its interactions in maintaining muscle physiology and energy homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-ENO3 antibody (ab232759)
All lanes: Western blot - Anti-ENO3 antibody (ab232759) at 3 µg/mL
Lane 1: Pig skeletal muscle lysate
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3: Pig heart lysate
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
SecondaryAll lanes: HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO3 antibody (ab232759)
Formalin-fixed paraffin-embedded human brain tissue stained for ENO3 using ab232759 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO3 antibody (ab232759)
Formalin-fixed paraffin-embedded human stomach cancer tissue stained for ENO3 using ab232759 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab232759)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ENO3 using Anti-HC-II antibody ab232859 at 20μg/mL in ICC/IF.
Western blot - Anti-ENO3 antibody (ab232759)
All lanes: Western blot - Anti-ENO3 antibody (ab232759) at 3 µg/mL
All lanes: Recombinant human ENO3 protein
Predicted band size: 47 kDa
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