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AB204433

Anti-ENO4 antibody

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(1 Publication)

Rabbit Polyclonal ENO4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ENO4 aa 50-150.

View Alternative Names

C10orf134, ENO4, Enolase 4, 2-phospho-D-glycerate hydro-lyase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ENO4 antibody (AB204433)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENO4 antibody (AB204433)

Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling ENO4 with ab204433 at 4 μg/mL.

Western blot - Anti-ENO4 antibody (AB204433)
  • WB

Supplier Data

Western blot - Anti-ENO4 antibody (AB204433)

All lanes:

Western blot - Anti-ENO4 antibody (ab204433) at 1/100 dilution

Lane 1:

RT4 lysate

Lane 2:

U251 MG lysate

Predicted band size: 69 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO4 antibody (AB204433)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO4 antibody (AB204433)

Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue labeling ENO4 with ab204433 at 1/50 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ENO4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

A6NNW6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENO4 an abbreviation for Enolase 4 is an enzyme that participates in the glycolytic pathway. This protein also known as testis-specific enolase has a mass of approximately 50 kDa. ENO4 expresses mainly in the testis although low levels appear in other tissues. Its functional role involves catalyzing the reversible conversion of 2-phosphoglycerate to phosphoenolpyruvate an essential step in glycolysis which plays an important role in energy production.
Biological function summary

ENO4 is important for energy metabolism within cells particularly in spermatogenesis. During this process ENO4 contributes to the development and maturation of sperm cells by ensuring efficient energy supply. While not a primary component of any large protein complexes ENO4 interacts with other glycolytic enzymes and requires co-factors like magnesium ions for its enzymatic activity. Its specificity and expression pattern make it distinctive among the enolase family members.

Pathways

ENO4 plays a significant role in the glycolytic pathway one of the central energy-producing processes in cells. This protein connects functionally with other glycolytic enzymes like phosphogluconate dehydrogenase and pyruvate kinase. These interactions ensure the sequential breakdown of glucose to generate ATP which is important for cell proliferation and energy maintenance especially in the context of spermatogenesis.

ENO4 has associations with male infertility and certain metabolic disorders. Due to its specific expression in the testis and role in sperm development alterations in ENO4 expression or function can lead to abnormalities in sperm production affecting male fertility. Moreover disruptions in glycolytic enzymes including ENO4 may contribute to metabolic syndromes although studies often focus on more common glycolytic enzymes. Understanding its interaction with related glycolytic proteins such as phosphogluconate dehydrogenase helps in clarifying its involvement in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be required for sperm motility and function.
See full target information ENO4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2203628119 PubMed36201541

2022

Succinyl-CoA-based energy metabolism dysfunction in chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Takada,Satoshi Maekawa,Takaaki Furihata,Naoya Kakutani,Daiki Setoyama,Koji Ueda,Hideo Nambu,Hikaru Hagiwara,Haruka Handa,Yoshizuki Fumoto,Soichiro Hata,Tomoka Masunaga,Arata Fukushima,Takashi Yokota,Dongchon Kang,Shintaro Kinugawa,Hisataka Sabe
View all publications
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Product promise

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