Anti-eNOS antibody [3/eNOS/NOS Type III] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [3/eNOS/NOS Type III] - BSA and Azide free (AB282129)

This data was developed using ab282109 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling eNOS with ab282109 at 1/2000 dilution (0.518 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in endothelial cells of human spleen. The section was incubated with ab282109 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [3/eNOS/NOS Type III] - BSA and Azide free (AB282129)

This data was developed using ab282109 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling eNOS with ab282109 at 1/2000 dilution (0.518 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in endothelial cells of mouse spleen. The section was incubated with ab282109 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• WB

Supplier Data

Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] - BSA and Azide free (AB282129)

This data was developed using ab282109, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Negative control : HeLa (PMID : 19559671).

Exposure time : 37 seconds.

All lanes:

Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] (<a href='/en-us/products/primary-antibodies/enos-antibody-3-enos-nos-type-iii-ab282109'>ab282109</a>) at 1/2000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

Ea.Hy926 (Human somatic cell hybrid endothelial), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

• WB

Supplier Data

Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] - BSA and Azide free (AB282129)

This data was developed using ab282109, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] (<a href='/en-us/products/primary-antibodies/enos-antibody-3-enos-nos-type-iii-ab282109'>ab282109</a>) at 1/5000 dilution

All lanes:

bEnd.3 (mouse brain endothelioma), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false