Anti-eNOS antibody [EPR19296]

• IP

Supplier Data

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (AB199956)

eNOS was immunoprecipitated from 1 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199956 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199956 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate 10 μg (Input).

Lane 2 : ab199956 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199956 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)

Predicted band size: 133 kDa

false

• WB

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID : 15210449 & 15936740).

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 17855661 & 11004215).

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 2 hours at 20 µg

Lane 3:

Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 6 hours at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Exposure Time : Lane1 : 40 seconds; Lane2 : 180 seconds

The expression profile observed in HeLa is consistent with the literature (PMID : 19925457).
Negative control : HeLa (PMID : 19925457)

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

• WB

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1, 2, 3, 4, 6 and 7 : 30 seconds; Lane 5 : 2 minutes.

eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID : 15210449 & 15936740).

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false

• WB

Supplier Data

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 second; Lane 2 : 3 minutes.

All lanes:

Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human eNOS protein (His-DDDDK tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-enos-protein-tagged-ab198066'>ab198066</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human iNOS protein (His-DDDDK tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-inos-protein-ab135010'>ab135010</a>) at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

false