Rabbit Recombinant Monoclonal eNOS antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 43 publications.
Images
![Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img163066.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS antibody [EPR19296] (AB199956)")
![Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img149320.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS antibody [EPR19296] (AB199956)")
![Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img137015.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS antibody [EPR19296] (AB199956)")
![Immunoprecipitation - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--immunoprecipitation-img50042.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-eNOS antibody [EPR19296] (AB199956)")
![Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img22731.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS antibody [EPR19296] (AB199956)")
Publications
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- Investigative ophthalmology & visual science 64:32023Diabetes Modulates Iodothyronine Deiodinase 2 Expression in the Mouse Retina: A Role for Thyroid Hormone in the Pathogenesis of Diabetic Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReena Bapputty,Hima Sapa,Miyagi Masaru,Rose A Gubitosi-KlugPubMed 38038617
- Lipids in health and disease 22:1892023High-fat stimulation induces atrial neural remodeling by reducing NO production via the CRIF1/eNOS/P21 axi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAn Zhang,Huilin Li,Qiyuan Song,Yansong Cui,Yujiao Zhang,Ximin Wang,Zhan Li,Yinglong HouPubMed 37932729
- Journal of cellular physiology 238:2827-28402023Mutual regulation between GDF11 and TET2 prevents senescence of mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaming Gao,Hao Wang,Junyan Shen,Xiaojing Liu,Xiaoqi Zhu,Ce Huang,Gongchen Li,Yao Sun,Zhongmin Liu,Yi Eve Sun,Hailiang LiuPubMed 37801347
- Science advances 9:eade55842023Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYizheng Wu,Shuying Shen,Jiaxin Chen,Weiyu Ni,Qinxin Wang,Hongyi Zhou,Junxin Chen,Haitao Zhang,Zixuan Mei,Xuewu Sun,Panyang Shen,Zhiwei Jie,Wenbin Xu,Zhenghua Hong,Yan Ma,Kefan Wang,Shuanglin Wan,Hongfei Wu,Ziang Xie,An Qin,Shunwu FanPubMed 36753544
- Journal of cardiovascular pharmacology 81:150-1642023HDAC Inhibitors Alleviate Uric Acid-Induced Vascular Endothelial Cell Injury by Way of the HDAC6/FGF21/PI3K/AKT Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaihao Wang,Youhong Zhang,Min Zhou,Yipeng Du,Peixin Li,Chang Guan,Zheng HuangPubMed 36607630
- Physiological reports 11:e155492023β3 adrenergic agonism: A novel pathway which improves right ventricular-pulmonary arterial hemodynamics in pulmonary arterial hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeyvan Karimi Galougahi,Yunjia Zhang,Vivian Kienzle,Chia-Chi Liu,Lake-Ee Quek,Sanjay Patel,Edmund Lau,Rachael L Cordina,Gemma A Figtree,David S CelermajerPubMed 36597221
- International journal of molecular sciences 23:2022The Protective Effects on Ischemia-Reperfusion Injury Mechanisms of the Thoracic Aorta in Daurian Ground Squirrels () over the Torpor-Arousal Cycle of Hibernation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuting Han,Weilan Miao,Ziwei Hao,Ning An,Yingyu Yang,Ziwen Zhang,Jiayu Chen,Kenneth B Storey,Etienne Lefai,Hui ChangPubMed 36142152
- ESC heart failure 9:2259-22712022LncRNA HOXA11-AS promotes vascular endothelial cell injury in atherosclerosis by regulating the miR-515-5p/ROCK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Gao,Xiao-Chen Wang,Zhi-Dan Luo,Guang-Quan Hu,Meng-Qing Ma,Yi Liang,Bang-Long Xu,Xian-He LinPubMed 35578440
- Frontiers in pharmacology 13:8386472022Radix Astragalus Polysaccharide Accelerates Angiogenesis by Activating AKT/eNOS to Promote Nerve Regeneration and Functional Recovery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeyi Zhang,Jinsheng Huang,Shuang Hao,Jingchao Zhang,Nan ZhouPubMed 35431954
- Aging 14:2695-27192022Effects of caloric overload before caloric restriction in the murine heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Maldonado,Jianying Chen,Huiqin Duan,Shuling Zhou,Lujun Yang,Mazhar Ali Raja,Tianhua Huang,Gu Jiang,Ying ZhongPubMed 35347086
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background. |
Species Rat | Dilution info 1/1000 | Notes We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background. |
Species Human | Dilution info 1/1000 | Notes We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background. |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Alternative names
Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
Recommended products
Rabbit Recombinant Monoclonal eNOS antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 43 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19296
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
- Biological function summary
ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.
- Pathways
ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.
- Associated diseases and disorders
ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img163066.jpg "Western blot - Anti-eNOS antibody [EPR19296] (ab199956)")
Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
Blocking/Dilution buffer: 5% NFDM/TBST.
eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID: 15210449 & 15936740).
All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution
Lane 1: Human fetal heart lysate at 20 µg
Lane 2: Human fetal kidney lysate at 20 µg
Lane 3: Human fetal liver lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
Exposure time: 3min
![Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img149320.jpg "Western blot - Anti-eNOS antibody [EPR19296] (ab199956)")
Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1, 2, 3, 4, 6 and 7: 30 seconds; Lane 5: 2 minutes.
eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID: 15210449 & 15936740).
All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Mouse spleen lysate at 10 µg
Lane 5: Rat brain lysate at 10 µg
Lane 6: Rat heart lysate at 10 µg
Lane 7: Rat spleen lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
![Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img137015.jpg "Western blot - Anti-eNOS antibody [EPR19296] (ab199956)")
Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
Exposure Time: Lane1: 40 seconds; Lane2: 180 seconds
The expression profile observed in HeLa is consistent with the literature (PMID: 19925457).
Negative control: HeLa (PMID: 19925457)All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
![Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--immunoprecipitation-img50042.jpg "Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)")
Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)
eNOS was immunoprecipitated from 1 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199956 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199956 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate 10 μg (Input).
Lane 2: ab199956 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199956 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)
Predicted band size: 133 kDa
![Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img22731.jpg "Western blot - Anti-eNOS antibody [EPR19296] (ab199956)")
Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:17855661 & 11004215).
All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/5000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 2 hours at 20 µg
Lane 3: Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 6 hours at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
Exposure time: 15s
![Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img78490.jpg "Western blot - Anti-eNOS antibody [EPR19296] (ab199956)")
Western blot - Anti-eNOS antibody [EPR19296] (ab199956)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.
All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution
Lane 1: Western blot - Recombinant Human eNOS protein (Tagged) (Recombinant Human eNOS protein (Tagged) ab198066) at 0.01 µg
Lane 2: Western blot - Recombinant Human iNOS protein (Recombinant Human iNOS protein ab135010) at 0.05 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
![Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail](https://content.abcam.com/products/images/enos-antibody-epr19296-ab199956--western-blot-img78490.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS antibody [EPR19296] (ab199956)")
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