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Rabbit Recombinant Monoclonal eNOS antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 43 publications.


Images

Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail
  • Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail
  • Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail
  • Immunoprecipitation - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail
  • Western blot - Anti-eNOS antibody [EPR19296] (AB199956), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.

Species

Rat

Dilution info

1/1000

Notes

We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.

Species

Human

Dilution info

1/1000

Notes

We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13CLacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Alternative names

Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, cNOS, eNOS, NOSIII, NOS3

Recommended products

Rabbit Recombinant Monoclonal eNOS antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 43 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19296

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail

    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID: 15210449 & 15936740).

    All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1: Human fetal heart lysate at 20 µg

    Lane 2: Human fetal kidney lysate at 20 µg

    Lane 3: Human fetal liver lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 133 kDa

    Observed band size: 140 kDa

    Exposure time: 3min

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail

    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2, 3, 4, 6 and 7: 30 seconds; Lane 5: 2 minutes.

    eNOS is shown to be expressed in platelets, placenta, liver and kidney (UniProt entry P29474). It also plays an important role in the cardiovascular system. (PMID: 15210449 & 15936740).

    All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: Mouse spleen lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: Rat heart lysate at 10 µg

    Lane 7: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 133 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail

    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)

    Exposure Time: Lane1: 40 seconds; Lane2: 180 seconds

    The expression profile observed in HeLa is consistent with the literature (PMID: 19925457).
    Negative control: HeLa (PMID: 19925457)

    All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 133 kDa

    Observed band size: 140 kDa

  • Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail

    Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)

    eNOS was immunoprecipitated from 1 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199956 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199956 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (Input).

    Lane 2: ab199956 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199956 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-eNOS antibody [EPR19296] (ab199956)

    Predicted band size: 133 kDa

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail

    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:17855661 & 11004215).

    All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/5000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 2 hours at 20 µg

    Lane 3: Whole cell lysate from HepG2 cells treated with 10 μg/ml LPS for 6 hours at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 133 kDa

    Observed band size: 140 kDa

    Exposure time: 15s

  • Western blot - Anti-eNOS antibody [EPR19296] (ab199956), expandable thumbnail

    Western blot - Anti-eNOS antibody [EPR19296] (ab199956)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

    All lanes: Western blot - Anti-eNOS antibody [EPR19296] (ab199956) at 1/1000 dilution

    Lane 1: Western blot - Recombinant Human eNOS protein (Tagged) (Recombinant Human eNOS protein (Tagged) ab198066) at 0.01 µg

    Lane 2: Western blot - Recombinant Human iNOS protein (Recombinant Human iNOS protein ab135010) at 0.05 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 133 kDa

    Observed band size: 140 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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