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Rabbit Recombinant Multiclonal eNOS antibody. Carrier free. Suitable for mIHC, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (AB317583), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (AB317583), expandable thumbnail
  • Western blot - Anti-eNOS antibody [RM1181] - BSA and Azide free (AB317583), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [RM1181] - BSA and Azide free (AB317583), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [RM1181] - BSA and Azide free (AB317583), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Not recommended
Tested
Rat
Expected
Expected
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13CLacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Alternative names

Recommended products

Rabbit Recombinant Multiclonal eNOS antibody. Carrier free. Suitable for mIHC, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

RM1181

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for Mouse-IHC.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab317583 is the carrier-free version of Anti-eNOS antibody [RM1181] ab317582.

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.

Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

Associated diseases and disorders

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

  • Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach labelling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/500 (B) and CD34 with Anti-CD34 antibody [EPR27432-54] ab315802 at 1/2000 dilution (C). Opal Polymer HRP Ms + Rb was used as a secondary antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-eNOS (magenta; Opal™520), anti-CD34 (green; Opal™570) on human stomach.

    Panel B: anti-eNOS staining endothelium in human stomach.

    Panel C: anti-CD34 staining endothelium in human stomach.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-eNOS antibody [RM1181] ab317582 and Anti-CD34 antibody [EPR27432-54] ab315802 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium labelling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/500 (B) and CD34 with Anti-CD34 antibody [EPR27432-54] ab315802 at 1/2000 dilution (C). Opal Polymer HRP Ms + Rb was used as a secondary antibody. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-eNOS (magenta; Opal™520), anti-CD34 (green; Opal™570) on human endometrium.

    Panel B: anti-eNOS staining endothelium in human endometrium.

    Panel C: anti-CD34 staining endothelium in human endometrium.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-eNOS antibody [RM1181] ab317582 and Anti-CD34 antibody [EPR27432-54] ab315802 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Western blot - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Western blot - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Negative controls: HeLa (PMID:19559671) and RAW 264.7 (PMID: 18607531).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16195740 and PMID: 11331296).

    This antibody was tested on fresh and frozen HUVEC and bEnd.3 whole Cell Lysate.

    The Lysates in lanes 6-7 were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1-3: 15 seconds Lane 4-5: 59 seconds Lane 6: 26 seconds Lane 7: 180 seconds

    All lanes: Western blot - Anti-eNOS antibody [RM1181] (Anti-eNOS antibody [RM1181] ab317582) at 1/1000 dilution

    Lanes 1 and 6: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 2: EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate at 20 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 4 and 7: bEnd.3 (mouse brain endothelial cell) whole cell lysate at 20 µg

    Lane 5: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 35 kDa, 60 kDa, 100 kDa, 140 kDa, 36 kDa

    Negative controls: HeLa (PMID:19559671) and RAW 264.7 (PMID: 18607531).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16195740 and PMID: 11331296).

    This antibody was tested on fresh and frozen HUVEC and bEnd.3 whole Cell Lysate.

    The Lysates in lanes 6-7 were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1-3: 15 seconds Lane 4-5: 59 seconds Lane 6: 26 seconds Lane 7: 180 seconds

  • Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelial cell) cells labelling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/100 (5.16 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic and membranous staining in bEnd.3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: RAW 264.7 (PMID: 18607531)

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/100 (5.16 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic and membranous staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: HeLa (PMID: 19559671)

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Western blot - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Low expression: skeletal muscle.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-eNOS antibody [RM1181] (Anti-eNOS antibody [RM1181] ab317582) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Human placenta tissue lysate at 20 µg

    Lane 3: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 35 kDa, 60 kDa, 100 kDa, 140 kDa, 36 kDa

    Exposure time: 180s

    Low expression: skeletal muscle.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/200 (2.58 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: no staining on rat skeletal muscle. The section was incubated with Anti-eNOS antibody [RM1181] ab317582 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/1000 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: almost no staining on human skeletal muscle. The section was incubated with Anti-eNOS antibody [RM1181] ab317582 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/200 (2.58 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelial cells in rat spleen. The section was incubated with Anti-eNOS antibody [RM1181] ab317582 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human endometrioid carcinoma tissue labeling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/1000 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelial cells in human endometrioid carcinoma. The section was incubated with Anti-eNOS antibody [RM1181] ab317582 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/1000 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human placenta. The section was incubated with Anti-eNOS antibody [RM1181] ab317582 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/1000 (0.516 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelial cells of human spleen. The section was incubated with Anti-eNOS antibody [RM1181] ab317582 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / bEnd.3(mouse brain endothelial cell, Right) cells labelling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: RAW 264.7

  • Flow Cytometry (Intracellular) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell, Left) / HUVEC(human umbilical vein endothelial cell, Right) cells labelling eNOS with Anti-eNOS antibody [RM1181] ab317582 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: HeLa

  • Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining eNOS with Anti-eNOS antibody [RM1181] ab317582 at a 1:1000 (1.004 ug/ml) dilution, Anti-eNOS antibody [RM1181] ab317582 anti-eNOS used at 1:500 (1.032 ug/ml) dilution and Anti-CD163 antibody [EPR19518] - BSA and Azide free ab213612 anti-CD163 used at a 1:8000 (0.13 ug/ml) dilution.

    Panel A: merged staining of anti-GLUT2 (magenta; Opal™690), anti-eNOS (green; Opal™520) and anti-CD163 (gray; Opal™570) on human liver.

    Panel B: anti-GLUT2 staining membrane of hepatocytes in human liver.

    Panel C: anti-eNOS staining endothelium in human liver.

    Panel D: anti-CD163 staining Kupffer cells in human liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] - BSA and Azide free ab260003, Anti-eNOS antibody [RM1181] ab317582 and Anti-CD163 antibody [EPR19518] - BSA and Azide free ab213612 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining eNOS with Anti-eNOS antibody [RM1181] ab317582 at a 1:500 (1.032 ug/ml) dilution, Anti-CD34 antibody [EPR27432-54] ab315802 anti-CD34 used at 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-eNOS (magenta; Opal™520), anti-CD34 (green; Opal™570) on human tonsil.

    Panel B: anti-eNOS staining endothelium in human tonsil.

    Panel C: anti-CD34 staining endothelium in human tonsil.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-eNOS antibody [RM1181] ab317582 and Anti-CD34 antibody [EPR27432-54] ab315802 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-eNOS antibody [RM1181] - BSA and Azide free (ab317583)

    This data was developed using Anti-eNOS antibody [RM1181] ab317582, the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining eNOS with Anti-eNOS antibody [RM1181] ab317582 at a 1:500 (1.032 ug/ml) dilution, Anti-CD34 antibody [EPR27432-54] ab315802 anti-CD34 used at 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-eNOS (magenta; Opal™520), anti-CD34 (green; Opal™570) on human liver.

    Panel B: anti-eNOS staining endothelium in human liver.

    Panel C: anti-CD34 staining endothelium in human liver.

    Panel D: Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-eNOS antibody [RM1181] ab317582 and Anti-CD34 antibody [EPR27432-54] ab315802 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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