AB184154

Anti-eNOS (phospho S1177) antibody

Name: Anti-eNOS (phospho S1177) antibody (ab184154) | Abcam
Brand: Abcam Limited
SKU: AB184154
Rating: 4 (2 reviews)

(2 Reviews)

(54 Publications)

Rabbit Polyclonal eNOS phospho S1177 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human NOS3 phospho S1177.

View Alternative Names

Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3

1 Images

Supplier Data

Western blot - Anti-eNOS (phospho S1177) antibody (AB184154)

5% SDS-PAGE

All lanes:

Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution

Lane 1:

Untreated THP-1 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts treated with 100nM PMA, 6 hr at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human NOS3 phospho S1177. The exact immunogen used to generate this antibody is proprietary information.

P29474

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.

Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

See full target information NOS3 phospho S1177

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Publications (54)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed39339680

2024

Combined Aerobic Exercise Training and Intake Reduces Arterial Stiffness through Enhanced Arterial Nitric Oxide Production in Obese Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Yamazaki,Shumpei Fujie,Kenichiro Inoue,Masataka Uchida,Motoyuki Iemitsu

Journal of the American Heart Association 13:e030054 PubMed38348774

2024

C1q/Tumor Necrosis Factor-Related Protein-9 Is a Novel Vasculoprotective Cytokine That Restores High Glucose-Suppressed Endothelial Progenitor Cell Functions by Activating the Endothelial Nitric Oxide Synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Hu,Wan Qu,Tao Zhang,Jianyi Feng,Xiaobian Dong,Ruqiong Nie,Junyu Chen,Xiaoqing Wang,Changnong Peng,Xiao Ke

Redox biology 67:102918 PubMed37812879

2023

Changes to insulin sensitivity in glucose clearance systems and redox following dietary supplementation with a novel cysteine-rich protein: A pilot randomized controlled trial in humans with type-2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

W M Peeters,M Gram,G J Dias,M C M Vissers,M B Hampton,N Dickerhof,A E Bekhit,M J Black,J Oxbøll,S Bayer,M Dickens,K Vitzel,P W Sheard,K M Danielson,L D Hodges,J C Brønd,J Bond,B G Perry,L Stoner,J Cornwall,D S Rowlands

Physiological research 72:S61-S72 PubMed37294119

2023

Effects of Iron Nanoparticles Administration on Ischemia/Reperfusion Injury in Isolated Hearts of Male Wistar Rats.

Applications

Unspecified application

Species

Unspecified reactive species

L Kindernay,K Ferenczyová,V Farkašova,M Barteková,I Bernátová,T Ravingerová

American journal of physiology. Heart and circulatory physiology 323:H688-H701 PubMed36018759

2022

ADAM17 cleaves the insulin receptor ectodomain on endothelial cells and causes vascular insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Thaysa Ghiarone,Jorge A Castorena-Gonzalez,Christopher A Foote,Francisco I Ramirez-Perez,Larissa Ferreira-Santos,Francisco J Cabral-Amador,Roger de la Torre,Rama R Ganga,Andrew A Wheeler,Camila Manrique-Acevedo,Jaume Padilla,Luis A Martinez-Lemus

Endocrinology 163: PubMed35974454

2022

Young Women Are Protected Against Vascular Insulin Resistance Induced by Adoption of an Obesogenic Lifestyle.

Applications

Unspecified application

Species

Unspecified reactive species

James A Smith,Rogerio N Soares,Neil J McMillan,Thomas J Jurrissen,Luis A Martinez-Lemus,Jaume Padilla,Camila Manrique-Acevedo

Molecular metabolism 64:101561 PubMed35944897

2022

Histone lysine demethylase inhibition reprograms prostate cancer metabolism and mechanics.

Applications

Unspecified application

Species

Unspecified reactive species

Ugo Chianese,Chiara Papulino,Eugenia Passaro,Tom Mj Evers,Mehrad Babaei,Antonella Toraldo,Tommaso De Marchi,Emma Niméus,Vincenzo Carafa,Maria Maddalena Nicoletti,Nunzio Del Gaudio,Nunzia Iaccarino,Antonio Randazzo,Dante Rotili,Antonello Mai,Salvatore Cappabianca,Alireza Mashaghi,Fortunato Ciardiello,Lucia Altucci,Rosaria Benedetti

Scientific reports 12:12681 PubMed35879337

2022

Empagliflozin mitigates endothelial inflammation and attenuates endoplasmic reticulum stress signaling caused by sustained glycocalyx disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Marc-Antoine Campeau,Richard L Leask

Theranostics 12:5172-5188 PubMed35836816

2022

Endothelial S1pr2 regulates post-ischemic angiogenesis via AKT/eNOS signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Caixia Zhou,Yashu Kuang,Qinyu Li,Yunhao Duan,Xiuxiang Liu,Jinnan Yue,Xiaoli Chen,Jie Liu,Yuzhen Zhang,Lin Zhang

Nature communications 13:646 PubMed35115501

2022

Schwann cell endosome CGRP signals elicit periorbital mechanical allodynia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco De Logu,Romina Nassini,Alan Hegron,Lorenzo Landini,Dane D Jensen,Rocco Latorre,Julia Ding,Matilde Marini,Daniel Souza Monteiro de Araujo,Paulina Ramírez-Garcia,Michael Whittaker,Jeffri Retamal,Mustafa Titiz,Alessandro Innocenti,Thomas P Davis,Nicholas Veldhuis,Brian L Schmidt,Nigel W Bunnett,Pierangelo Geppetti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eNOS (phospho S1177) antibody

Western blot - Anti-eNOS (phospho S1177) antibody (AB184154)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (54)

Product promise