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Rabbit Polyclonal eNOS phospho S1177 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 46 publications. Immunogen corresponding to Synthetic Peptide within Human NOS3 phospho S1177.


Images

Western blot - Anti-eNOS (phospho S1177) antibody (AB184154), expandable thumbnail
  • Western blot - Anti-eNOS (phospho S1177) antibody (AB184154), expandable thumbnail
  • Western blot - Anti-eNOS (phospho S1177) antibody (AB184154), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human NOS3 phospho S1177. The exact immunogen used to generate this antibody is proprietary information. Database link P29474

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Guinea pig
Predicted
Predicted
Pig
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Guinea pig, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Predicted
Predicted

Species

Guinea pig, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13CLacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Alternative names

Recommended products

Rabbit Polyclonal eNOS phospho S1177 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 46 publications. Immunogen corresponding to Synthetic Peptide within Human NOS3 phospho S1177.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human NOS3 phospho S1177. The exact immunogen used to generate this antibody is proprietary information. Database link P29474
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.

Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

Associated diseases and disorders

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.

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3 product images

  • Western blot - Anti-eNOS (phospho S1177) antibody (ab184154), expandable thumbnail

    Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)

    5% SDS-PAGE

    All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution

    Lane 1: Jurkat whole cell extract at 30 µg

    Lane 2: Raji whole cell extract at 30 µg

    Lane 3: NCI-H929 whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-eNOS (phospho S1177) antibody (ab184154), expandable thumbnail

    Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)

    All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/1000 dilution

    All lanes: HUVEC whole cell extract at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-eNOS (phospho S1177) antibody (ab184154), expandable thumbnail

    Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)

    5% SDS-PAGE

    All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution

    Lane 1: Untreated THP-1 whole cell extracts at 30 µg

    Lane 2: THP-1 whole cell extracts treated with 100nM PMA, 6 hr at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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