Rabbit Polyclonal eNOS phospho S1177 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 46 publications. Immunogen corresponding to Synthetic Peptide within Human NOS3 phospho S1177.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Guinea pig | Predicted | Predicted |
Pig | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Guinea pig, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Guinea pig, Pig | Dilution info - | Notes - |
Select an associated product type
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13CLacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
Rabbit Polyclonal eNOS phospho S1177 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 46 publications. Immunogen corresponding to Synthetic Peptide within Human NOS3 phospho S1177.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.
ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.
ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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5% SDS-PAGE
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1: Jurkat whole cell extract at 30 µg
Lane 2: Raji whole cell extract at 30 µg
Lane 3: NCI-H929 whole cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/1000 dilution
All lanes: HUVEC whole cell extract at 50 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
5% SDS-PAGE
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1: Untreated THP-1 whole cell extracts at 30 µg
Lane 2: THP-1 whole cell extracts treated with 100nM PMA, 6 hr at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
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