Rabbit Polyclonal eNOS phospho S1177 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 46 publications. Immunogen corresponding to Synthetic Peptide within Human NOS3 phospho S1177.
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Publications
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- American journal of physiology. Heart and circulatory physiology 323:H688-H7012022ADAM17 cleaves the insulin receptor ectodomain on endothelial cells and causes vascular insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThaysa Ghiarone,Jorge A Castorena-Gonzalez,Christopher A Foote,Francisco I Ramirez-Perez,Larissa Ferreira-Santos,Francisco J Cabral-Amador,Roger de la Torre,Rama R Ganga,Andrew A Wheeler,Camila Manrique-Acevedo,Jaume Padilla,Luis A Martinez-LemusPubMed 36018759
- Endocrinology 163:2022Young Women Are Protected Against Vascular Insulin Resistance Induced by Adoption of an Obesogenic Lifestyle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames A Smith,Rogerio N Soares,Neil J McMillan,Thomas J Jurrissen,Luis A Martinez-Lemus,Jaume Padilla,Camila Manrique-AcevedoPubMed 35974454
- Molecular metabolism 64:1015612022Histone lysine demethylase inhibition reprograms prostate cancer metabolism and mechanics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUgo Chianese,Chiara Papulino,Eugenia Passaro,Tom Mj Evers,Mehrad Babaei,Antonella Toraldo,Tommaso De Marchi,Emma Niméus,Vincenzo Carafa,Maria Maddalena Nicoletti,Nunzio Del Gaudio,Nunzia Iaccarino,Antonio Randazzo,Dante Rotili,Antonello Mai,Salvatore Cappabianca,Alireza Mashaghi,Fortunato Ciardiello,Lucia Altucci,Rosaria BenedettiPubMed 35944897
- Theranostics 12:5172-51882022Endothelial S1pr2 regulates post-ischemic angiogenesis via AKT/eNOS signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaixia Zhou,Yashu Kuang,Qinyu Li,Yunhao Duan,Xiuxiang Liu,Jinnan Yue,Xiaoli Chen,Jie Liu,Yuzhen Zhang,Lin ZhangPubMed 35836816
- Nature communications 13:6462022Schwann cell endosome CGRP signals elicit periorbital mechanical allodynia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesco De Logu,Romina Nassini,Alan Hegron,Lorenzo Landini,Dane D Jensen,Rocco Latorre,Julia Ding,Matilde Marini,Daniel Souza Monteiro de Araujo,Paulina Ramírez-Garcia,Michael Whittaker,Jeffri Retamal,Mustafa Titiz,Alessandro Innocenti,Thomas P Davis,Nicholas Veldhuis,Brian L Schmidt,Nigel W Bunnett,Pierangelo GeppettiPubMed 35115501
- Molecular medicine (Cambridge, Mass.) 27:1562021Metformin alleviates the calcification of aortic valve interstitial cells through activating the PI3K/AKT pathway in an AMPK dependent way.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiao En,Huang Zeping,Wang Yuetang,Wang Xu,Wang WeiPubMed 34895136
- Molecular medicine reports 25:2021FGF21 attenuates high uric acid‑induced endoplasmic reticulum stress, inflammation and vascular endothelial cell dysfunction by activating Sirt1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Ouyang,Xiaoqin Zhao,Rongping Zhang,Jing Yang,Siyin Li,Daihua DengPubMed 34850960
- Journal of vascular research 58:311-3202021Nuclear Factor Kappa-B/Homeobox A9-Mediated Modulation of Leucine-Rich Repeat Flightless-Interacting Protein 1 Is Involved in Advanced Glycation End Product-Induced Endothelial Dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianqian Zhu,Songjie Hu,Yunjun He,Chenyang Qiu,Tianchi Chen,Yangyan He,Ziheng Wu,Lu Tian,Tao Shang,Yilang Xiang,Hongkun Zhang,Donglin LiPubMed 33882503
- Molecular medicine reports 23:2020Dickkopf‑1/cysteine‑rich angiogenic inducer 61 axis mediates palmitic acid‑induced inflammation and apoptosis of vascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Rong Gan,Ling Wei,Yan-Zhen Wang,Zong-Ke Kou,Tian-Xiang Liang,Guan-Waner Ding,Yan-Hong Ding,Ding-Xiong XiePubMed 33300071
- Cardiovascular drugs and therapy :2020Extracellular Vesicles Derived from Intermittent Hypoxia-Treated Red Blood Cells Impair Endothelial Function Through Regulating eNOS Phosphorylation and ET-1 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Peng et. al.PubMed 33242203
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NOS3 phospho S1177. The exact immunogen used to generate this antibody is proprietary information. Database link P29474
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Guinea pig | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Alternative names
Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
Recommended products
Rabbit Polyclonal eNOS phospho S1177 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 46 publications. Immunogen corresponding to Synthetic Peptide within Human NOS3 phospho S1177.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NOS3 phospho S1177. The exact immunogen used to generate this antibody is proprietary information. Database link P29474
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
- Biological function summary
ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.
- Pathways
ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.
- Associated diseases and disorders
ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)
5% SDS-PAGE
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1: Jurkat whole cell extract at 30 µg
Lane 2: Raji whole cell extract at 30 µg
Lane 3: NCI-H929 whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)
5% SDS-PAGE
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1: Untreated THP-1 whole cell extracts at 30 µg
Lane 2: THP-1 whole cell extracts treated with 100nM PMA, 6 hr at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody (ab184154) at 1/1000 dilution
All lanes: HUVEC whole cell extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
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