Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) is a rabbit monoclonal antibody that is used to detect eNOS in Western Blot, IP, Dot Blot. Suitable for Human, Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 20 publications
Images
![Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--western-blot-img78222.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)")
![Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--immunoprecipitation-img11621.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)")
![Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--dot-blot-img5733.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)")
![Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--western-blot-img91008.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)")
![Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--western-blot-img38816.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (AB215717)")
Publications
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- Scientific reports 13:224852023LncRNA SNHG8 regulates the migration and angiogenesis of pHUVECs induced by high glucose via the TRPM7/ERK signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZongcheng Fan,Xin Chen,Laicheng Wang,Jianjian Yu,Shunpeng Zhang,Changsheng Xu,Jinxiu Lin,Yunchai Lin,Feng PengPubMed 38110485
- Acta biochimica Polonica 70:843-8532023Modified Hongyu Decoction promotes wound healing by activating the VEGF/PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Xu,Wei-Hua Yang,Zhi-Wei Miao,Chun-Yu Zhang,Yi-Jia Cheng,Yang Chen,Jin-Gen Lu,Ning HePubMed 38051819
- PloS one 18:e02948482023Dexrazoxane does not mitigate early vascular toxicity induced by doxorubicin in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthias Bosman,Dustin N Krüger,Kasper Favere,Guido R Y De Meyer,Constantijn Franssen,Emeline M Van Craenenbroeck,Pieter-Jan GunsPubMed 38015959
- Science advances 9:eade55842023Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYizheng Wu,Shuying Shen,Jiaxin Chen,Weiyu Ni,Qinxin Wang,Hongyi Zhou,Junxin Chen,Haitao Zhang,Zixuan Mei,Xuewu Sun,Panyang Shen,Zhiwei Jie,Wenbin Xu,Zhenghua Hong,Yan Ma,Kefan Wang,Shuanglin Wan,Hongfei Wu,Ziang Xie,An Qin,Shunwu FanPubMed 36753544
- Journal of cardiovascular pharmacology 81:150-1642023HDAC Inhibitors Alleviate Uric Acid-Induced Vascular Endothelial Cell Injury by Way of the HDAC6/FGF21/PI3K/AKT Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaihao Wang,Youhong Zhang,Min Zhou,Yipeng Du,Peixin Li,Chang Guan,Zheng HuangPubMed 36607630
- Frontiers in physiology 13:9615342022Haptoglobin treatment contributes to regulating nitric oxide signal and reduces oxidative stress in the penis: A preventive treatment for priapism in sickle cell disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPamela da Silva Pereira,Dalila Andrade Pereira,Fabiano Beraldi Calmasini,Leonardo O Reis,Nathan Brinkman,Arthur L Burnett,Fernando Ferreira Costa,Fábio Henrique SilvaPubMed 36176769
- Journal of cellular and molecular medicine 26:5165-51802022Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Liu,Shuya Wang,Guangxin Wang,Mingming Zhao,Fengli Du,Kaiyuan Li,Lei Wang,Huihui Wu,Jiamin Chen,Yang Yang,Guohai SuPubMed 36071548
- Frontiers in endocrinology 13:9132962022NLRP3 downregulation enhances engraftment and functionality of adipose-derived stem cells to alleviate erectile dysfunction in diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Luo,Yaqian Peng,Xiongcai Zhou,Junhong Fan,Weihong Chen,Haibo Zhang,Anyang WeiPubMed 35937790
- Clinical science (London, England : 1979) 136:973-9872022Alpha7 nicotinic acetylcholine receptor mediates chronic nicotine inhalation-induced cardiopulmonary dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna K Whitehead,Nicholas D Fried,Zhen Li,Kandasamy Neelamegam,Charlotte S Pearson,Kyle B LaPenna,Thomas E Sharp,David J Lefer,Eric Lazartigues,Jason D Gardner,Xinping YuePubMed 35678315
- ESC heart failure 9:2259-22712022LncRNA HOXA11-AS promotes vascular endothelial cell injury in atherosclerosis by regulating the miR-515-5p/ROCK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Gao,Xiao-Chen Wang,Zhi-Dan Luo,Guang-Quan Hu,Meng-Qing Ma,Yi Liang,Bang-Long Xu,Xian-He LinPubMed 35578440
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Dot | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Expected | Tested |
| Synthetic peptide | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Alternative names
Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3
Recommended products
Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) is a rabbit monoclonal antibody that is used to detect eNOS in Western Blot, IP, Dot Blot. Suitable for Human, Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20991
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Dot Blot, in Human, Mouse samples.
What is the molecular weight of eNOS?
Anti-eNOS (phospho S1177) [EPR20991] (ab215717) specifically detects a band for eNOS (UniProt: P29474) at a molecular weight of 133kDa.
Recommended positive controls
WB: HUVEC (+/- VEGF or pervanadate) whole cell lysates; bEND.3 whole cell lysate.IP: HUVEC treated with VEGF whole cell lysate.Dot blot: eNOS (phospho S1177) peptide.
Trusted by the scientific community
Anti-eNOS (phospho S1177) [EPR20991] (ab215717) was first used in a scientific publication in 2018 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR20991] also available for your convenience:
ab215717, Carrier free - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free ab230158
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
- Biological function summary
ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.
- Pathways
ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.
- Associated diseases and disorders
ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--western-blot-img78222.jpg "Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)")
Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) at 1/1000 dilution
Lane 1: bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg
Lane 2: bEND.3 (mouse brain endothelioma cell line) treated with alkaline phosphatase for 1 hour, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 133 kDa
Observed band size: 140 kDa
Exposure time: 2min
![Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--immunoprecipitation-img11621.jpg "Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)")
Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)
eNOS (phospho S1177) was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate with ab215717 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215717 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: HUVEC treated with VEGF whole cell lysate 10 μg (Input).
Lane 2: ab215717 IP in HUVEC treated with VEGF whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab215717 in HUVEC treated with VEGF whole cell lysate.Exposure time: 30 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).
All lanes: Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)
Developed using the ECL technique.
Predicted band size: 133 kDa
Observed band size: 140 kDa
![Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--dot-blot-img5733.jpg "Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)")
Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)
Dot blot analysis of eNOS (phospho S1177) labeled with ab215717 at 1/1000 dilution.
Lane 1: eNOS (phospho S1177) peptide.
Lane 2: eNOS non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Exposure time: 1 minute.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--western-blot-img91008.jpg "Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)")
Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)
Blocking/Dilution buffer: 5% NFDM/TBST.
The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) at 1/1000 dilution
Lane 1: HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 10 µg
Lane 2: HUVEC (human umbilical vein endothelial cell line) treated with 100 uM pervanadate for 10 min, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 133 kDa
Observed band size: 140 kDa
Exposure time: 3min
![Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717), expandable thumbnail](https://content.abcam.com/products/images/enos-phospho-s1177-antibody-epr20991-ab215717--western-blot-img38816.jpg "Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)")
Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717)
Blocking/Dilution buffer: 5% NFDM/TBST.
The 60 kDa band may be a cleaved form of eNOS (PMID: 16195740).
All lanes: Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (ab215717) at 1/1000 dilution
Lane 1: Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 10 µg
Lane 2: HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 133 kDa
Observed band size: 140 kDa
Exposure time: 3min
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