AB230158

Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal eNOS phospho S1177 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Synthetic peptide samples. Cited in 5 publications.

View Alternative Names

Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS, NOS3

3 Images

Supplier Data

Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (AB230158)

eNOS (phospho S1177) was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate with ab215717 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215717 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HUVEC treated with VEGF whole cell lysate 10 μg (Input).
Lane 2 : ab215717 IP in HUVEC treated with VEGF whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab215717 in HUVEC treated with VEGF whole cell lysate.

Exposure time : 30 seconds.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

The 60 kDa band may be a cleaved form of eNOS (PMID : 16195740).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215717).

All lanes:

Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] (<a href='/en-us/products/primary-antibodies/enos-phospho-s1177-antibody-epr20991-ab215717'>ab215717</a>)

Predicted band size: 133 kDa

Observed band size: 140 kDa

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Supplier Data

Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (AB230158)

Blocking/Dilution buffer : 5% NFDM/TBST.

The 60 kDa band may be a cleaved form of eNOS (PMID : 16195740).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215717).

All lanes:

Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] (<a href='/en-us/products/primary-antibodies/enos-phospho-s1177-antibody-epr20991-ab215717'>ab215717</a>) at 1/1000 dilution

Lane 1:

Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 10 µg

Lane 2:

HUVEC (human umbilical vein endothelial cell line) treated with 10 ng/ml VEGF for 5 min, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 133 kDa

Observed band size: 140 kDa

true

Exposure time: 3min

Supplier Data

Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (AB230158)

Dot blot analysis of eNOS (phospho S1177) labeled with ab215717 at 1/1000 dilution.

Lane 1 : eNOS (phospho S1177) peptide.
Lane 2 : eNOS non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Exposure time : 1 minute.

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215717).

Unconjugated

Anti-eNOS (phospho S1177) antibody [EPR20991]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20991

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IP, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab230158 is the carrier-free version of ab215717.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.

Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed : 1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.. Isoform eNOS13C. Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

See full target information NOS3 phospho S1177

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed37892970

2023

Enhancing Muscle Intracellular Ca Homeostasis and Glucose Uptake: Passive Pulsatile Shear Stress Treatment in Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Arkady Uryash,Jordan Umlas,Alfredo Mijares,Jose A Adams,Jose R Lopez

Journal of clinical biochemistry and nutrition 72:171-182 PubMed36936876

2023

Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: and experiments and a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Shakya Supriya,Ryoko Ushikoshi-Nakayama,Tomoe Yamazaki,Daisuke Omagari,Keiko Aota,Hiroko Inoue,Naoyuki Matsumoto,Ichiro Saito

Scientific reports 11:21789 PubMed34750450

2021

The effects of bathing in neutral bicarbonate ion water.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoe Yamazaki,Ryoko Ushikoshi-Nakayama,Supriya Shakya,Daisuke Omagari,Naoyuki Matsumoto,Chiyoko Nukuzuma,Tomoko Komatsu,Masaichi Chang-Il Lee,Hiroko Inoue,Ichiro Saito

Frontiers in pharmacology 12:646240 PubMed34177571

2021

Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Liyun Zhang,Juan Chen,Lianhua Yan,Qin He,Han Xie,Manhua Chen

Journal of cellular and molecular medicine 23:5349-5359 PubMed31131539

2019

Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyang Wang,Jinyu Pan,Dian Liu,Mingjun Zhang,Xiaowei Li,Jingjing Tian,Ming Liu,Tao Jin,Fengshuang An

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free

Immunoprecipitation - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (AB230158)

Western blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (AB230158)

Dot Blot - Anti-eNOS (phospho S1177) antibody [EPR20991] - BSA and Azide free (AB230158)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise