Anti-eNOS (phospho S632) antibody [M232]

• WB

Unknown

Western blot - Anti-eNOS (phospho S632) antibody [M232] (AB76199)

The 120 kDa band may be a truncated form of eNOS.

All lanes:

Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution

Lane 1:

human umbilical vein endothelialcells, untreated

Lane 2:

human umbilical vein endothelialcells, treated with lambda phosphatase

Predicted band size: 133 kDa

Observed band size: 120 kDa,140 kDa

false

• WB

CiteAb

Western blot - Anti-eNOS (phospho S632) antibody [M232] (AB76199)

eNOS (phospho S632) western blot using anti-eNOS (phospho S632) antibody [M232] ab76199. Publication image and figure legend from Gupta, A., Anjomani-Virmouni, S., et al., 2017, Mol Cell, PubMed 28306514.

ab76199 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76199 please see the product overview.

PARK2 Depletion Leads to Enhanced S-nitrosylation and Ubiquitination of PTEN(A) Anti-PTEN immunoprecipitates (IP) derived from MYC-tagged-transfected PTEN HCT116 cells expressing GFP or PARK2 shRNA.(B) Fluorometric measurement of S-nitrosylated PTEN between shGFP and shPARK2 HCT116 cells. SNO-PTEN was assessed by NO release, causing the conversion of DAN to the fluorescent compound NAT (p = 0.009).(C and D) Immunoblot analysis of (C) whole-cell lysates and (D) anti-PTEN immunoprecipitates (IP) derived from HA-ubiquitin (Ub) and Myc-tagged PTEN-transfected HCT116 cells expressing GFP or PARK2 shRNA. Where indicated, cells were treated with MG132 (10 μM) for 6 hr before collection.(E–G) Immunoblot analysis and anti-PTEN immunoprecipitates derived from (E) Myc-tagged WT or C83S mutant PTEN-transfected HCT116 cells expressing GFP or PARK2 shRNA; (F) WT PTEN-transfected shGFP and shPARK2 HCT116 cells, 48 hr post-transfection with scrambled or AMPK α1 and AMPK α2 siRNAs; and (G) parental HCT116 treated (or not treated) with the allosteric AMPK activator 991 for 5 hr (20 μM) following 2 hr serum starvation (left) or with 25-mM glucose-containing DMEM (middle) for 6 hr or with oligomycin (5 μM) for 2 hr. Data are represented as mean ± SEM.See also Figures S5 and S6.

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