AB210751

Anti-ENOX2/tNOX antibody

Name: Anti-ENOX2/tNOX antibody (ab210751) | Abcam
Brand: Abcam Limited
SKU: AB210751

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(1 Publication)

Rabbit Polyclonal ENOX2/tNOX antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ENOX2 aa 400 to C-terminus.

View Alternative Names

COVA1, ENOX2, Ecto-NOX disulfide-thiol exchanger 2, APK1 antigen, Cytosolic ovarian carcinoma antigen 1, Tumor-associated hydroquinone oxidase, tNOX

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ENOX2/tNOX antibody (AB210751)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENOX2/tNOX antibody (AB210751)

Immunofluorescence staining of C6 cells stained for ENOX2/tNOX with ab210751 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

Supplier Data

Western blot - Anti-ENOX2/tNOX antibody (AB210751)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-ENOX2/tNOX antibody (ab210751)

Lane 1:

MCF-7 cell lysate at 25 µg

Lane 2:

THP-1 cell lysate at 25 µg

Lane 3:

SW480 cell lysate at 25 µg

Lane 4:

Jurkat cell lysate at 25 µg

Lane 5:

SKOV3 cell lysate at 25 µg

Lane 6:

Mouse liver tissue lysate at 25 µg

Lane 7:

Mouse kidney tissue lysate at 25 µg

Lane 8:

Mouse ovary tissue lysate at 25 µg

Lane 9:

Rat liver tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 70 kDa

true

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ENOX2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16206

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENOX2 also known as tumor-associated NADH oxidase or tNOX is a protein involved in cellular processes including growth and apoptosis. It has a molecular mass of approximately 46 kDa and exhibits unique activity patterns cycling with a 24-minute period. ENOX2 is expressed mostly in cancer cells but not in normal cells which makes it a potential biomarker for various types of cancer. This expression pattern offers a significant advantage for targeted cancer therapies focusing efforts on treating malignant cells while sparing healthy tissues.

Biological function summary

This enzyme plays a role in electron transport and cell surface NADH oxidase activity. ENOX2 forms part of a complex involved in the regulation of cellular energy and growth directly impacting cellular proliferation rates. It contributes to the maintenance of extracellular environments that support cancer cell survival and proliferation. Its activity parallels growth rates in cancer cells further implicating its function in tumorigenesis and oncogenic processes.

Pathways

ENOX2 operates within the NADH/NAD+ redox system and impacts the pathways that govern cellular metabolism and energy flow. It interacts with proteins in the oxidative phosphorylation pathway which is critical for ATP production and cellular energy regulation. ENOX2 links to proteins such as cytochrome c oxidase and its influence extends to pathways associated with glycolytic processes and mitochondrial function illustrating its central part in metabolic reprogramming in cancer cells.

ENOX2 is strongly associated with cancer serving as both a diagnostic marker and a potential therapeutic target. Its cancer-specific expression pattern exposes its linkage to oncogenic pathways. ENOX2 is connected to other proteins implicated in cancer such as HER2/neu playing a role in breast cancer. Additionally it relates to neurodegenerative disorders where alterations in cellular metabolism contribute to disease progression although the precise mechanisms remain under investigation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.

See full target information ENOX2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical genetics 98:493-498 PubMed32779182

2020

Homozygous TAF1C variants are associated with a novel childhood-onset neurological phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Oula Knuutinen,Angela Pyle,Maria Suo-Palosaari,Jennifer Duff,Tawfiq Froukh,Anna-Elina Lehesjoki,Salla M Kangas,James Cassidy,Latifa Maraqa,Riikka Keski-Filppula,Hannaleena Kokkonen,Johanna Uusimaa,Rita Horvath,Päivi Vieira

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENOX2/tNOX antibody

Immunocytochemistry/ Immunofluorescence - Anti-ENOX2/tNOX antibody (AB210751)

Western blot - Anti-ENOX2/tNOX antibody (AB210751)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise