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Rabbit Recombinant Monoclonal ENPP1/PC1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (AB314552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (AB314552), expandable thumbnail
  • Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (AB314552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (AB314552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (AB314552), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow CytIPICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Mouse
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels (PubMed:10352096, PubMed:11004006, PubMed:12082181, PubMed:22510396, PubMed:25260930, PubMed:9662402). PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals (PubMed:10352096, PubMed:11004006, PubMed:12082181, PubMed:19419305, PubMed:22510396, PubMed:25260930, PubMed:25479107, PubMed:26910915, PubMed:30111653, PubMed:35147247, PubMed:9662402). Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP (PubMed:11027689, PubMed:1647027, PubMed:23027977, PubMed:8223581). May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling (PubMed:1647027). Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect (PubMed:30111653). Appears to modulate insulin sensitivity (By similarity). Also involved in melanogenesis (By similarity). Also able to hydrolyze 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production (PubMed:25344812). 2',3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2',3'-cGAMP hydrolysis is therefore unclear (By similarity). Not able to hydrolyze the 2',3'-cGAMP linkage isomer 3',3'-cGAMP (By similarity).

Alternative names

Npps, Pc1, Pdnp1, Enpp1, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Alkaline phosphodiesterase I, Lymphocyte antigen 41, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Ly-41, NPPase

Recommended products

Rabbit Recombinant Monoclonal ENPP1/PC1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28388-32
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314552 is the carrier-free version of Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ENPP1 also known as PC-1 is a type II transmembrane glycoprotein with a molecular weight of approximately 130 kDa. It is expressed in various tissues including the liver kidney and bone. ENPP1 exhibits the enzymatic activity of nucleotide pyrophosphatase/phosphodiesterase which hydrolyzes ATP to AMP and diphosphate. This activity is important for the regulation of extracellular nucleotide levels and mineralization processes. Researchers often utilize the ENPP1 assay to study its function and activity.

Biological function summary

ENPP1 significantly influences bone mineralization and soft tissue calcification. It interacts with proteins like ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) impacting cellular uptake of calcified materials. The protein is also involved in the regulatory networks responsible for controlling insulin signaling impacting metabolic processes. ENPP1 may form complexes with other proteins which can finely tune its biological activity and effects.

Pathways

ENPP1 plays a role in the insulin signaling pathway and the pyrophosphate pathway. Via its participation in insulin signaling ENPP1 regulates glucose metabolism and influences insulin sensitivity. It affects pathways related to mineral metabolism where it interacts with proteins like ENPP3. By regulating extracellular pyrophosphate levels ENPP1 contributes to inorganic phosphate homeostasis and influences skeletal development and maintenance.

Associated diseases and disorders

ENPP1 has associations with type 2 diabetes and generalized arterial calcification of infancy (GACI). Its role in type 2 diabetes relates to its ability to inhibit insulin receptor function therefore contributing to insulin resistance. ENPP1 also connects to proteins involved in vascular calcification such as matrix Gla protein (MGP) in the context of GACI. These associations highlight the importance of ENPP1 in metabolic and calcification disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling ENPP1/PC1 with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 at 1/1000 (0.515 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: weak staining on rat skeletal muscle. The section was incubated with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling ENPP1/PC1 with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 at 1/1000 (0.515 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression: weak staining on mouse cardiac muscle. The section was incubated with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: skeletal muscle, heart, testis.
    The identity of the higher MW band at approximately 250 kDa is unknown.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] (Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 3: Mouse heart tissue lysate at 20 µg

    Lane 4: Mouse testis tissue lysate at 20 µg

    Lane 5: Rat liver tissue lysate at 20 µg

    Lane 6: Rat kidney tissue lysate at 20 µg

    Lane 7: Rat skeletal muscle tissue lysate at 20 µg

    Lane 8: Rat heart tissue lysate at 20 µg

    Lane 9: Rat testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 130 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ENPP1/PC1 with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 at 1/1000 (0.515 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat liver. The section was incubated with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ENPP1/PC1 with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 at 1/1000 (0.515 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney.The section was incubated with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ENPP1/PC1 with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 at 1/1000 (0.515 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver (PMID: 3262656). The section was incubated with Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with mouse ENPP2 and ENPP3.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution

    All lanes: Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] (Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 7 µg

    Lane 2: 293T cells transfected with a mouse ENPP1 expression vector containing a His-tag, whole cell lysate at 7 µg

    Lane 3: 293T cells transfected with a mouse ENPP2 expression vector containing a His-tag, whole cell lysate at 7 µg

    Lane 4: 293T cells transfected with a mouse ENPP3 expression vector containing a His-tag, whole cell lysate at 7 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 130 kDa

    Exposure time: 8s

  • Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: F9.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: Lane 1: 37 seconds;
    Lanes 2-3: 180 seconds.

    All lanes: Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] (Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 130 kDa

  • Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552), expandable thumbnail

    Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] - BSA and Azide free (ab314552)

    This data was developed using Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The identity of the higher MW band at approximately 250 kDa is unknown.

    All lanes: Western blot - Anti-ENPP1/PC1 antibody [EPR28388-32] (Anti-ENPP1/PC1 antibody [EPR28388-32] ab314551) at 1/1000 dilution

    Lane 1: Mouse kidney tissue lysate at 20 µg

    Lane 2: Mouse dorsal galion tissue lysate at 20 µg

    Lane 3: Rat dorsal galion tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 130 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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