AB320008

Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free

BOND RX™ Validated
Recombinant
RabMAb
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Multiclonal ENPP1/PC1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Transfected cell lysate - Mouse, Human, Mouse, Rat samples.

View Alternative Names

Npps, Pc1, Pdnp1, Enpp1, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Alkaline phosphodiesterase I, Lymphocyte antigen 41, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Ly-41, NPPase, M6S1, NPPS, PC1, PDNP1, ENPP1, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Alkaline phosphodiesterase I, Membrane component chromosome 6 surface marker 1, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, NPPase

15 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ENPP1/PC1 with ab320007 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on human skeletal muscle.
The section was incubated with ab320007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling ENPP1/PC1 with ab320007 at 1/1000 (0.506 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing mainly membranous staining in HepG2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : DLD-1.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling ENPP1/PC1 with ab320007 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ENPP1/PC1 with ab320007 at 1/1000 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human liver.
The section was incubated with ab320007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

ENPP1/PC1 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab320007 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab320007 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : ab320007 IP in Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab320007 in Huh7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-ENPP1/PC1 antibody [RM2066] (<a href='/en-us/products/primary-antibodies/enpp1-pc1-antibody-rm2066-ab320007'>ab320007</a>) at 1/30 dilution

All lanes:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 76s

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling ENPP1/PC1 with ab320007 at 1/500 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on mouse skeletal muscle.
The section was incubated with ab320007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling ENPP1/PC1 with ab320007 at 1/500 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on rat skeletal muscle.
The section was incubated with ab320007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ENPP1/PC1 with ab320007 at 1/500 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse liver.
The section was incubated with ab320007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ENPP1/PC1 with ab320007 at 1/500 (0.506 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat liver.
The section was incubated with ab320007 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle.

The identity of the bands higher than 250 kDa and lower than 15 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ENPP1/PC1 antibody [RM2066] (<a href='/en-us/products/primary-antibodies/enpp1-pc1-antibody-rm2066-ab320007'>ab320007</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : DLD-1, Caco-2 (PMID : 31978347), SK-BR-3 (PMID : 23861746).

The identity of the bands higher than 250 kDa and lower than 25 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 10 seconds; Lanes 2-5 : 37 seconds.

All lanes:

Western blot - Anti-ENPP1/PC1 antibody [RM2066] (<a href='/en-us/products/primary-antibodies/enpp1-pc1-antibody-rm2066-ab320007'>ab320007</a>) at 1/1000 dilution

Lane 1:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa,36 kDa

false

Supplier Data

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle.

The identity of the bands higher than 250 kDa and lower than 50 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ENPP1/PC1 antibody [RM2066] (<a href='/en-us/products/primary-antibodies/enpp1-pc1-antibody-rm2066-ab320007'>ab320007</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 70s

Supplier Data

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENPP1/PC1 antibody [RM2066] (<a href='/en-us/products/primary-antibodies/enpp1-pc1-antibody-rm2066-ab320007'>ab320007</a>) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Mouse dorsal ganglion tissue lysate at 20 µg

Lane 3:

Rat dorsal ganglion tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa

false

Exposure time: 81s

Supplier Data

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : F9.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ENPP1/PC1 antibody [RM2066] (<a href='/en-us/products/primary-antibodies/enpp1-pc1-antibody-rm2066-ab320007'>ab320007</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

This data was developed using ab320007, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse ENPP2 and ENPP3.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-ENPP1/PC1 antibody [RM2066] (<a href='/en-us/products/primary-antibodies/enpp1-pc1-antibody-rm2066-ab320007'>ab320007</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a mouse ENPP1 expression vector containing a His-tag, whole cell lysate at 10 µg

Lane 3:

293T cells transfected with a mouse ENPP2 expression vector containing a His-tag, whole cell lysate at 10 µg

Lane 4:

293T cells transfected with a mouse ENPP3 expression vector containing a His-tag, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 8s

Unconjugated

Anti-ENPP1/PC1 antibody [RM2066]

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM2066

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, IP, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

ab320008 is the carrier-free version of ab320007.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENPP1 also known as PC-1 is a type II transmembrane glycoprotein with a molecular weight of approximately 130 kDa. It is expressed in various tissues including the liver kidney and bone. ENPP1 exhibits the enzymatic activity of nucleotide pyrophosphatase/phosphodiesterase which hydrolyzes ATP to AMP and diphosphate. This activity is important for the regulation of extracellular nucleotide levels and mineralization processes. Researchers often utilize the ENPP1 assay to study its function and activity.

Biological function summary

ENPP1 significantly influences bone mineralization and soft tissue calcification. It interacts with proteins like ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1) impacting cellular uptake of calcified materials. The protein is also involved in the regulatory networks responsible for controlling insulin signaling impacting metabolic processes. ENPP1 may form complexes with other proteins which can finely tune its biological activity and effects.

Pathways

ENPP1 plays a role in the insulin signaling pathway and the pyrophosphate pathway. Via its participation in insulin signaling ENPP1 regulates glucose metabolism and influences insulin sensitivity. It affects pathways related to mineral metabolism where it interacts with proteins like ENPP3. By regulating extracellular pyrophosphate levels ENPP1 contributes to inorganic phosphate homeostasis and influences skeletal development and maintenance.

ENPP1 has associations with type 2 diabetes and generalized arterial calcification of infancy (GACI). Its role in type 2 diabetes relates to its ability to inhibit insulin receptor function therefore contributing to insulin resistance. ENPP1 also connects to proteins involved in vascular calcification such as matrix Gla protein (MGP) in the context of GACI. These associations highlight the importance of ENPP1 in metabolic and calcification disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels (PubMed : 10352096, PubMed : 11004006, PubMed : 12082181, PubMed : 22510396, PubMed : 25260930, PubMed : 9662402). PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals (PubMed : 10352096, PubMed : 11004006, PubMed : 12082181, PubMed : 19419305, PubMed : 22510396, PubMed : 25260930, PubMed : 25479107, PubMed : 26910915, PubMed : 30111653, PubMed : 35147247, PubMed : 9662402). Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP (PubMed : 11027689, PubMed : 1647027, PubMed : 23027977, PubMed : 8223581). May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling (PubMed : 1647027). Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect (PubMed : 30111653). Appears to modulate insulin sensitivity (By similarity). Also involved in melanogenesis (By similarity). Also able to hydrolyze 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production (PubMed : 25344812). 2',3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced; the role of 2',3'-cGAMP hydrolysis is therefore unclear (By similarity). Not able to hydrolyze the 2',3'-cGAMP linkage isomer 3',3'-cGAMP (By similarity).

See full target information Enpp1

Additional targets

ENPP1

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Immunocytochemistry/ Immunofluorescence - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Flow Cytometry (Intracellular) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Immunoprecipitation - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Western blot - Anti-ENPP1/PC1 antibody [RM2066] - BSA and Azide free (AB320008)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise