AB238670

Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free

BOND RX™ Validated
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(1 Publication)

Mouse Monoclonal ENPP2/ATX antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ATX, PDNP2, ENPP2, Autotaxin, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, E-NPP 2, LysoPLD

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (AB238670)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (AB238670)

IHC image of ENPP2/ATX staining in Human Tonsil FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77104, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

This data was developed using the same antibody clone in a different formulation containing PBS, Azide and arginine (ab77104).

Unconjugated

Anti-ENPP2/ATX antibody [1F8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F8

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab238670 is the carrier-free version of ab77104.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP2 protein also known as autotaxin (ATX) with a mass of approximately 102 kDa mainly manifests as a secreted enzyme with lysophospholipase D activity. ENPP2 or ATX-001 widely expresses in various tissues predominantly in the brain adipose tissue and placenta. It catalyzes the hydrolysis of lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline playing an important role in LPA signaling. ENPP2/ATX-001 is a valuable component in the study of lipid signaling pathways.

Biological function summary

ATX protein influences cell proliferation migration and survival. It does not operate as part of a complex but directly impacts the biological processes previously mentioned due to its enzymatic function. It acts significantly during embryonic development and wound healing. The products of its catalysis LPA engage in various signaling pathways essential for these processes.

Pathways

ENPP2 integrates into the lysophospholipid and phospholipase pathways. It plays an important role in the LPA receptor signaling pathway contributing to various physiological and pathological processes. Proteins related to ENPP2 through these pathways include LPA receptors such as LPAR1 and LPAR2 with which it interacts closely to mediate cell signaling roles and phospholipase A2 which provides substrate for its enzymatic activity.

ENPP2 links strongly with cancer and fibrotic diseases. Its enzymatic activity generates LPA which can enhance tumor progression by promoting cell proliferation and migration. This activity associates ENPP2 with oncogenic processes. Additionally LPA signaling stands connected to fibrotic disorders where it contributes to tissue remodeling and fibrosis. Its overactivity or dysregulation might result in such pathological states associating indirectly with growth factor proteins like TGFB1 which are involved in tissue fibrosis and cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed : 12354767, PubMed : 14500380, PubMed : 15769751, PubMed : 26371182, PubMed : 27754931). Its major substrate is lysophosphatidylcholine (PubMed : 12176993, PubMed : 14500380, PubMed : 27754931). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed : 14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed : 12176993, PubMed : 15769751). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed : 11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed : 1733949). May have a role in induction of parturition (PubMed : 12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18 : 2 and 20 : 4 fatty acids (PubMed : 21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed : 21393252).

See full target information ENPP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxins 14: PubMed35050979

2021

Molecular Characterization and Functional Analysis of the Nattectin-like Toxin from the Venomous Fish .

Applications

Unspecified application

Species

Unspecified reactive species

Monica Lopes-Ferreira,Ines Sosa-Rosales,Pedro Ismael Silva Junior,Katia Conceicao,Adolfo Luis Almeida Maleski,Leticia Balan-Lima,Geonildo Rodrigo Disner,Carla Lima

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (AB238670)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Multiplex Kits

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise