Mouse Monoclonal ENPP2/ATX antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (AB238670), expandable thumbnail](https://content.abcam.com/products/images/enpp2-atx-antibody-1f8-bsa-and-azide-free-ab238670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26832.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (AB238670)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC | IHC-P | WB | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
2 products for Alternative Version
Target data
Function
Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:12354767, PubMed:14500380, PubMed:15769751, PubMed:26371182, PubMed:27754931). Its major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:14500380, PubMed:27754931). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:12176993, PubMed:15769751). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed:21393252).
Alternative names
ATX, PDNP2, ENPP2, Autotaxin, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, E-NPP 2, LysoPLD
Recommended products
Mouse Monoclonal ENPP2/ATX antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 1F8
- Purity
- IgG fraction
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238670 is the carrier-free version of Anti-ENPP2/ATX antibody [1F8] ab77104.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ENPP2 protein also known as autotaxin (ATX) with a mass of approximately 102 kDa mainly manifests as a secreted enzyme with lysophospholipase D activity. ENPP2 or ATX-001 widely expresses in various tissues predominantly in the brain adipose tissue and placenta. It catalyzes the hydrolysis of lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline playing an important role in LPA signaling. ENPP2/ATX-001 is a valuable component in the study of lipid signaling pathways.
- Biological function summary
ATX protein influences cell proliferation migration and survival. It does not operate as part of a complex but directly impacts the biological processes previously mentioned due to its enzymatic function. It acts significantly during embryonic development and wound healing. The products of its catalysis LPA engage in various signaling pathways essential for these processes.
- Pathways
ENPP2 integrates into the lysophospholipid and phospholipase pathways. It plays an important role in the LPA receptor signaling pathway contributing to various physiological and pathological processes. Proteins related to ENPP2 through these pathways include LPA receptors such as LPAR1 and LPAR2 with which it interacts closely to mediate cell signaling roles and phospholipase A2 which provides substrate for its enzymatic activity.
- Associated diseases and disorders
ENPP2 links strongly with cancer and fibrotic diseases. Its enzymatic activity generates LPA which can enhance tumor progression by promoting cell proliferation and migration. This activity associates ENPP2 with oncogenic processes. Additionally LPA signaling stands connected to fibrotic disorders where it contributes to tissue remodeling and fibrosis. Its overactivity or dysregulation might result in such pathological states associating indirectly with growth factor proteins like TGFB1 which are involved in tissue fibrosis and cancer.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (ab238670), expandable thumbnail](https://content.abcam.com/products/images/enpp2-atx-antibody-1f8-bsa-and-azide-free-ab238670--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26832.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (ab238670)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] - BSA and Azide free (ab238670)
IHC image of ENPP2/ATX staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-ENPP2/ATX antibody [1F8] ab77104, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This data was developed using the same antibody clone in a different formulation containing PBS, Azide and arginine (Anti-ENPP2/ATX antibody [1F8] ab77104).
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