Rabbit Polyclonal ENPP2/ATX antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP2 aa 500-850.
Images
Publications
Filter by
- Frontiers in molecular biosciences 8:7459902021Hepatitis B Virus Promotes Hepatocellular Carcinoma Progression Synergistically With Hepatic Stellate Cells Facilitating the Expression and Secretion of ENPP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanyu Deng,Fu Chen,Ziyu Zhou,Yipei Huang,Junlong Lin,Fapeng Zhang,Gang Xiao,Chaoqun Liu,Chao Liu,Leibo XuPubMed 34805271
- Journal of molecular endocrinology 63:113-1212019Adipose tissue-derived autotaxin causes cardiomyopathy in obese mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYousheng Xu,Yongshun Wang,Jingjin Liu,Wei Cao,Lili Li,Hongwei Du,Enbo Zhan,Ruoxi Zhang,Huimin Liu,Maoen Xu,Tao Chen,Yilin Qu,Bo YuPubMed 31247591
- Stem cells (Dayton, Ohio) 34:551-642016Autotaxin Regulates Maintenance of Ovarian Cancer Stem Cells through Lysophosphatidic Acid-Mediated Autocrine Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun Jin Seo et. al.PubMed 26800320
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ENPP2 aa 500-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q13822
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:12354767, PubMed:14500380, PubMed:15769751, PubMed:26371182, PubMed:27754931). Its major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:14500380, PubMed:27754931). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:12176993, PubMed:15769751). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed:21393252).
Alternative names
ATX, PDNP2, ENPP2, Autotaxin, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, E-NPP 2, LysoPLD
Recommended products
Rabbit Polyclonal ENPP2/ATX antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP2 aa 500-850.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ENPP2 aa 500-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q13822
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ENPP2 protein also known as autotaxin (ATX) with a mass of approximately 102 kDa mainly manifests as a secreted enzyme with lysophospholipase D activity. ENPP2 or ATX-001 widely expresses in various tissues predominantly in the brain adipose tissue and placenta. It catalyzes the hydrolysis of lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline playing an important role in LPA signaling. ENPP2/ATX-001 is a valuable component in the study of lipid signaling pathways.
- Biological function summary
ATX protein influences cell proliferation migration and survival. It does not operate as part of a complex but directly impacts the biological processes previously mentioned due to its enzymatic function. It acts significantly during embryonic development and wound healing. The products of its catalysis LPA engage in various signaling pathways essential for these processes.
- Pathways
ENPP2 integrates into the lysophospholipid and phospholipase pathways. It plays an important role in the LPA receptor signaling pathway contributing to various physiological and pathological processes. Proteins related to ENPP2 through these pathways include LPA receptors such as LPAR1 and LPAR2 with which it interacts closely to mediate cell signaling roles and phospholipase A2 which provides substrate for its enzymatic activity.
- Associated diseases and disorders
ENPP2 links strongly with cancer and fibrotic diseases. Its enzymatic activity generates LPA which can enhance tumor progression by promoting cell proliferation and migration. This activity associates ENPP2 with oncogenic processes. Additionally LPA signaling stands connected to fibrotic disorders where it contributes to tissue remodeling and fibrosis. Its overactivity or dysregulation might result in such pathological states associating indirectly with growth factor proteins like TGFB1 which are involved in tissue fibrosis and cancer.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-ENPP2/ATX antibody (ab137590)
7.5% SDS-PAGE
All lanes: Western blot - Anti-ENPP2/ATX antibody (ab137590) at 1/1000 dilution
All lanes: Human Cerebrospinal Fluid at 30 µg
Predicted band size: 98 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (ab137590)
SK-N-SH cells stained for Autotaxin (green) using ab137590 at 1/500 dilution in ICC/IF.
Western blot - Anti-ENPP2/ATX antibody (ab137590)
7.5% SDS PAGE
All lanes: Western blot - Anti-ENPP2/ATX antibody (ab137590) at 1/500 dilution
Lane 1: HepG2 whole cell lysate at 30 µg
Lane 2: MOLT4 whole cell lysate at 30 µg
Predicted band size: 98 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com