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AB137590

Anti-ENPP2/ATX antibody

1

(1 Review)

|

(3 Publications)

Rabbit Polyclonal ENPP2/ATX antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP2 aa 500-850.

View Alternative Names

ATX, PDNP2, ENPP2, Autotaxin, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, E-NPP 2, LysoPLD

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (AB137590)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (AB137590)

SK-N-SH cells stained for Autotaxin (green) using ab137590 at 1/500 dilution in ICC/IF.

Western blot - Anti-ENPP2/ATX antibody (AB137590)
  • WB

Supplier Data

Western blot - Anti-ENPP2/ATX antibody (AB137590)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-ENPP2/ATX antibody (ab137590) at 1/1000 dilution

All lanes:

Human Cerebrospinal Fluid at 30 µg

Predicted band size: 98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ENPP2 aa 500-850. The exact immunogen used to generate this antibody is proprietary information.

Q13822

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP2 protein also known as autotaxin (ATX) with a mass of approximately 102 kDa mainly manifests as a secreted enzyme with lysophospholipase D activity. ENPP2 or ATX-001 widely expresses in various tissues predominantly in the brain adipose tissue and placenta. It catalyzes the hydrolysis of lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline playing an important role in LPA signaling. ENPP2/ATX-001 is a valuable component in the study of lipid signaling pathways.
Biological function summary

ATX protein influences cell proliferation migration and survival. It does not operate as part of a complex but directly impacts the biological processes previously mentioned due to its enzymatic function. It acts significantly during embryonic development and wound healing. The products of its catalysis LPA engage in various signaling pathways essential for these processes.

Pathways

ENPP2 integrates into the lysophospholipid and phospholipase pathways. It plays an important role in the LPA receptor signaling pathway contributing to various physiological and pathological processes. Proteins related to ENPP2 through these pathways include LPA receptors such as LPAR1 and LPAR2 with which it interacts closely to mediate cell signaling roles and phospholipase A2 which provides substrate for its enzymatic activity.

ENPP2 links strongly with cancer and fibrotic diseases. Its enzymatic activity generates LPA which can enhance tumor progression by promoting cell proliferation and migration. This activity associates ENPP2 with oncogenic processes. Additionally LPA signaling stands connected to fibrotic disorders where it contributes to tissue remodeling and fibrosis. Its overactivity or dysregulation might result in such pathological states associating indirectly with growth factor proteins like TGFB1 which are involved in tissue fibrosis and cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed : 12354767, PubMed : 14500380, PubMed : 15769751, PubMed : 26371182, PubMed : 27754931). Its major substrate is lysophosphatidylcholine (PubMed : 12176993, PubMed : 14500380, PubMed : 27754931). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed : 14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed : 12176993, PubMed : 15769751). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed : 11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed : 1733949). May have a role in induction of parturition (PubMed : 12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18 : 2 and 20 : 4 fatty acids (PubMed : 21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed : 21393252).
See full target information ENPP2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 8:745990 PubMed34805271

2021

Hepatitis B Virus Promotes Hepatocellular Carcinoma Progression Synergistically With Hepatic Stellate Cells Facilitating the Expression and Secretion of ENPP2.

Applications

Unspecified application

Species

Unspecified reactive species

Wanyu Deng,Fu Chen,Ziyu Zhou,Yipei Huang,Junlong Lin,Fapeng Zhang,Gang Xiao,Chaoqun Liu,Chao Liu,Leibo Xu

Journal of molecular endocrinology 63:113-121 PubMed31247591

2019

Adipose tissue-derived autotaxin causes cardiomyopathy in obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yousheng Xu,Yongshun Wang,Jingjin Liu,Wei Cao,Lili Li,Hongwei Du,Enbo Zhan,Ruoxi Zhang,Huimin Liu,Maoen Xu,Tao Chen,Yilin Qu,Bo Yu

Stem cells (Dayton, Ohio) 34:551-64 PubMed26800320

2016

Autotaxin Regulates Maintenance of Ovarian Cancer Stem Cells through Lysophosphatidic Acid-Mediated Autocrine Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Jin Seo,Yang Woo Kwon,Il Ho Jang,Dae Kyoung Kim,Soo In Lee,Eun Jung Choi,Ki-Hyung Kim,Dong-Soo Suh,Jeong Hee Lee,Kyung Un Choi,Jae Won Lee,Hyuck Jun Mok,Kwang Pyo Kim,Hirotaka Matsumoto,Junken Aoki,Jae Ho Kim
View all publications
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