AB227306

Anti-ENPP2/ATX antibody

Name: Anti-ENPP2/ATX antibody (ab227306) | Abcam
Brand: Abcam Limited
SKU: AB227306

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(1 Publication)

Rabbit Polyclonal ENPP2/ATX antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP2.

View Alternative Names

ATX, PDNP2, ENPP2, Autotaxin, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, E-NPP 2, LysoPLD

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (AB227306)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (AB227306)

NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for ENPP2/ATX (green) using ab227306 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.

Blue : Hoechst 33342 staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ENPP2. The exact immunogen used to generate this antibody is proprietary information.

Q13822

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP2 protein also known as autotaxin (ATX) with a mass of approximately 102 kDa mainly manifests as a secreted enzyme with lysophospholipase D activity. ENPP2 or ATX-001 widely expresses in various tissues predominantly in the brain adipose tissue and placenta. It catalyzes the hydrolysis of lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline playing an important role in LPA signaling. ENPP2/ATX-001 is a valuable component in the study of lipid signaling pathways.

Biological function summary

ATX protein influences cell proliferation migration and survival. It does not operate as part of a complex but directly impacts the biological processes previously mentioned due to its enzymatic function. It acts significantly during embryonic development and wound healing. The products of its catalysis LPA engage in various signaling pathways essential for these processes.

Pathways

ENPP2 integrates into the lysophospholipid and phospholipase pathways. It plays an important role in the LPA receptor signaling pathway contributing to various physiological and pathological processes. Proteins related to ENPP2 through these pathways include LPA receptors such as LPAR1 and LPAR2 with which it interacts closely to mediate cell signaling roles and phospholipase A2 which provides substrate for its enzymatic activity.

ENPP2 links strongly with cancer and fibrotic diseases. Its enzymatic activity generates LPA which can enhance tumor progression by promoting cell proliferation and migration. This activity associates ENPP2 with oncogenic processes. Additionally LPA signaling stands connected to fibrotic disorders where it contributes to tissue remodeling and fibrosis. Its overactivity or dysregulation might result in such pathological states associating indirectly with growth factor proteins like TGFB1 which are involved in tissue fibrosis and cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed : 12354767, PubMed : 14500380, PubMed : 15769751, PubMed : 26371182, PubMed : 27754931). Its major substrate is lysophosphatidylcholine (PubMed : 12176993, PubMed : 14500380, PubMed : 27754931). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed : 14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed : 12176993, PubMed : 15769751). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed : 11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed : 1733949). May have a role in induction of parturition (PubMed : 12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18 : 2 and 20 : 4 fatty acids (PubMed : 21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed : 21393252).

See full target information ENPP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 15:1082-1094 PubMed38553795

2024

Circ-POSTN promotes the progression and reduces radiosensitivity in esophageal cancer by regulating the miR-876-5p/FYN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Alan Chu,Chen Sun,Zongwen Liu,Shijia Liu,Mengxi Li,Rui Song,Lanlan Gan,Yongtai Wang,Ruitai Fan

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ENPP2/ATX antibody

Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody (AB227306)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise