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AB314041

Anti-ENPP3/B10 antibody [3B2] - Human IgG1 (Chimeric)

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Mouse Monoclonal ENPP3/B10 antibody. Suitable for Flow Cyt, FuncS and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENPP3.

View Alternative Names

CD203c, PDNP3, ENPP3, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, NPP3, Alkaline phosphodiesterase I, Dinucleoside polyphosphatase, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I beta, Phosphodiesterase I/nucleotide pyrophosphatase 3, NPPase, PD-Ibeta

3 Images
Flow Cytometry - Anti-ENPP3/B10 antibody [3B2] - Human IgG1 (Chimeric) (AB314041)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ENPP3/B10 antibody [3B2] - Human IgG1 (Chimeric) (AB314041)

Untransfected HEK293T cells (green line) and transfected human ENPP3/B10 HEK293T stable cells (pink line) were stained with ab314041 (2µg/1x10E6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Functional Studies - Anti-ENPP3/B10 antibody [3B2] - Human IgG1 (Chimeric) (AB314041)
  • FuncS

Supplier Data

Functional Studies - Anti-ENPP3/B10 antibody [3B2] - Human IgG1 (Chimeric) (AB314041)

The binding activity of human ENPP3/B10 with ab314041 Activity : Measured by its binding ability in a functional ELISA. Immobilized human ENPP3/B10 at 2 μg/mL can bind ab314041, the EC50 is 2.151-2.492 ng/mL

Functional Studies - Anti-ENPP3/B10 antibody [3B2] - Human IgG1 (Chimeric) (AB314041)
  • FuncS

Supplier Data

Functional Studies - Anti-ENPP3/B10 antibody [3B2] - Human IgG1 (Chimeric) (AB314041)

The binding activity of Macaca fascicularis ENPP3/B10 with ab314041 Activity : Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3/B10 at 2 μg/mL can bind ab314041, the EC50 is 3.313-4.724 ng/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B2

Isotype

IgG1

Carrier free

No

Reacts with

Human, Macaque monkey

Applications

Flow Cyt, FuncS

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ENPP3.

O14638

Specificity

We only have data for transfected human/ Macaque samples, we currently have no endogenous data.

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP3/B10 protein also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.
Biological function summary

ENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.

Pathways

This enzyme engages in the purinergic signaling pathway and can influence the MAPK/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK/ERK signaling.

ENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed : 29717535, PubMed : 9344668). Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (By similarity). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well (PubMed : 12846830). In addition, displays an alkaline phosphodiesterase activity in vitro (PubMed : 11342463).
See full target information ENPP3
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