AB233777

Anti-ENPP3/B10 antibody [4C1H2]

Name: Anti-ENPP3/B10 antibody [4C1H2] (ab233777) | Abcam
Brand: Abcam Limited
SKU: AB233777
Price: 410 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Mouse Monoclonal ENPP3/B10 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP3 aa 1-200.

View Alternative Names

CD203c, PDNP3, ENPP3, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, NPP3, Alkaline phosphodiesterase I, Dinucleoside polyphosphatase, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I beta, Phosphodiesterase I/nucleotide pyrophosphatase 3, NPPase, PD-Ibeta

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody [4C1H2] (AB233777)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody [4C1H2] (AB233777)

Paraffin-embedded human renal cancer tissue stained for ENPP3/B10 using ab233777 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-ENPP3/B10 antibody [4C1H2] (AB233777)

Expected MW is 13.6 kDa.

All lanes:

Western blot - Anti-ENPP3/B10 antibody [4C1H2] (ab233777) at 1/500 dilution

All lanes:

Human recombinant ENPP3/B10 (AA: extra 45-163) protein.

Predicted band size: 100 kDa

false

Flow Cytometry - Anti-ENPP3/B10 antibody [4C1H2] (AB233777)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ENPP3/B10 antibody [4C1H2] (AB233777)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling ENPP3/B10 using ab233777 at 1/200 dilution (green) and the negative control (red).

Supplier Data

Western blot - Anti-ENPP3/B10 antibody [4C1H2] (AB233777)

All lanes:

Western blot - Anti-ENPP3/B10 antibody [4C1H2] (ab233777) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

ENPP3/B10 (AA: extra 45-163)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 100 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4C1H2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ENPP3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O14638

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Orangutan": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP3/B10 protein also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.

Biological function summary

ENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.

Pathways

This enzyme engages in the purinergic signaling pathway and can influence the MAPK/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK/ERK signaling.

ENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed : 29717535, PubMed : 9344668). Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (By similarity). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well (PubMed : 12846830). In addition, displays an alkaline phosphodiesterase activity in vitro (PubMed : 11342463).

See full target information ENPP3

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:848-856 PubMed38149831

2023

Hypomethylation of the promoter region contributes to the occurrence and development of ovarian endometriosis via the AKT/mTOR/4EBP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhen Qin,Yishuai Li,Yali Hao,Yan Li,Shan Kang

Frontiers in immunology 11:563699 PubMed33324395

2020

microRNA-630 Regulates Underglycosylated IgA1 Production in the Tonsils by Targeting TLR4 in IgA Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Liu,Mu-Yao Ye,Wen-Zhe Yan,Xiao-Fei Peng,Li-Yu He,You-Ming Peng

Science advances 6:eaay0888 PubMed32195342

2020

Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Sakamoto,Toru Komatsu,Rikiya Watanabe,Yi Zhang,Taiki Inoue,Mitsuyasu Kawaguchi,Hidehiko Nakagawa,Takaaki Ueno,Takuji Okusaka,Kazufumi Honda,Hiroyuki Noji,Yasuteru Urano

View all publications

style

left-column

style

left-column

style

right-column