AB189116

Anti-ENPP3/B10 antibody

Name: Anti-ENPP3/B10 antibody (ab189116) | Abcam
Brand: Abcam Limited
SKU: AB189116
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal ENPP3/B10 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ENPP3.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody (AB189116)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody (AB189116)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling ENPP3/B10 with ab189116 at 15 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody (AB189116)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling ENPP3/B10 with ab189116 at 15 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ENPP3. The exact immunogen used to generate this antibody is proprietary information.

O14638

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "15 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ENPP3/B10 protein also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.

Biological function summary

ENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.

Pathways

This enzyme engages in the purinergic signaling pathway and can influence the MAPK/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK/ERK signaling.

ENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed : 29717535, PubMed : 9344668). Also hydrolyzes extracellular 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production, and is therefore involved in the regulation of innate immune response (PubMed : 38749434). 2',3'-cGAMP appears to be further processed to GMP, AMP and inorganic phosphate (PubMed : 38749434). Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation (By similarity). Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well (PubMed : 12846830). In addition, displays an alkaline phosphodiesterase activity in vitro (PubMed : 11342463).

See full target information ENPP3

Alternative Names

CD203c, PDNP3, ENPP3, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, NPP3, Alkaline phosphodiesterase I, Dinucleoside polyphosphatase, Nucleotide diphosphatase, Nucleotide pyrophosphatase, Phosphodiesterase I beta, Phosphodiesterase I/nucleotide pyrophosphatase 3, NPPase, PD-Ibeta

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Osteoarthritis and cartilage 31:507-518 PubMed36370958

2022

Hypoxia and Wnt signaling inversely regulate expression of chondroprotective molecule ANP32A in articular cartilage.

Applications

Unspecified application

Species

Unspecified reactive species

J Quintiens,A De Roover,F M F Cornelis,A Escribano-Núñez,A Sermon,S Pazmino,S Monteagudo,R J Lories

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ENPP3/B10 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody (AB189116)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP3/B10 antibody (AB189116)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise