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AB137014

Anti-ENPP5 antibody [EPR8236]

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(2 Publications)

Rabbit Recombinant Monoclonal ENPP5 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

UNQ550/PRO1107, ENPP5, Ectonucleotide pyrophosphatase/phosphodiesterase family member 5, E-NPP 5, NPP-5

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ENPP5 antibody [EPR8236] (AB137014)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENPP5 antibody [EPR8236] (AB137014)

Immunofluorescent analysis of MCF7 cells labelling ENPP5 with ab137014 at 1/250 dilution.

Western blot - Anti-ENPP5 antibody [EPR8236] (AB137014)
  • WB

Unknown

Western blot - Anti-ENPP5 antibody [EPR8236] (AB137014)

All lanes:

Western blot - Anti-ENPP5 antibody [EPR8236] (ab137014) at 1/1000 dilution

Lane 1:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

Neuro-2a cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 55 kDa

false

OI-RD Scanning - Anti-ENPP5 antibody [EPR8236] (AB137014)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ENPP5 antibody [EPR8236] (AB137014)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8236

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENPP5 also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 5 is an enzyme that belongs to the ENPP family. It plays a role in the hydrolysis of phosphodiester bonds although specific substrates for ENPP5 remain less well-defined compared to other ENPPs. This protein has a molecular weight of approximately 49 kDa. ENPP5 is mainly expressed in tissues such as the brain and the kidneys suggesting it holds specialized functions in these areas.
Biological function summary

Researchers propose that ENPP5 contributes to nucleotide metabolism and signaling processes. Although ENPP5 is not directly part of a larger protein complex it functions alongside similar enzymes sharing common substrates and physiological roles. By maintaining balance in the extracellular nucleotide levels ENPP5 can influence processes such as cellular signaling potentially affecting cell growth and differentiation in its primary tissues.

Pathways

ENPP5 activity relates to purine metabolism and the signal transduction pathways that involve nucleotides. One primary pathway where it plays a role is the nucleotide catabolic process interacting indirectly with proteins such as ENPP1 and ENPP2 that manage pyrophosphate and phospholipid metabolism. ENPP5 by breaking down nucleotides influences signal transduction pathways like the P2Y receptor-mediated pathways which are important for various physiological functions.

ENPP5 has connections with conditions involving defective nucleotide metabolism or signaling imbalances. Researchers suggest possible links to neurological disorders due to its expression in the brain though exact implications require further study. ENPP5 alongside proteins like ENPP1 may show involvement in pathologies characterized by abnormal ectonucleotide levels. However more research is essential to establish clear associations with specific diseases such as neurodegenerative disorders or renal pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Can hydrolyze NAD but cannot hydrolyze nucleotide di- and triphosphates. Lacks lysopholipase D activity. May play a role in neuronal cell communication.
See full target information ENPP5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0303265 PubMed38739590

2024

Immortalized hepatocyte-like cells: A competent hepatocyte model for studying clinical HCV isolate infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyut Pewkliang,Piyanoot Thongsri,Phichaya Suthivanich,Nipa Thongbaiphet,Jiraporn Keatkla,Ekawat Pasomsub,Usanarat Anurathapan,Suparerk Borwornpinyo,Adisak Wongkajornsilp,Suradej Hongeng,Khanit Sa-Ngiamsuntorn

Cellular and molecular biology (Noisy-le-Grand, France) 69:180-188 PubMed38015522

2023

Identification of potential diagnostic biomarkers and immune infiltration features in diabetic foot ulcer by bioinformatics analysis and validation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohua Li,Biwu Chen,Yuanyuan Xu,Anbang Zhou,Biaoliang Wu
View all publications
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Product promise

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