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AB127897

Anti-ENPP6 antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal ENPP6 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP6 aa 1-250.

View Alternative Names

UNQ1889/PRO4334, ENPP6, Glycerophosphocholine cholinephosphodiesterase ENPP6, GPC-Cpde, Choline-specific glycerophosphodiester phosphodiesterase, Ectonucleotide pyrophosphatase/phosphodiesterase family member 6, E-NPP 6, NPP-6

1 Images
Western blot - Anti-ENPP6 antibody (AB127897)
  • WB

Unknown

Western blot - Anti-ENPP6 antibody (AB127897)

10% SDS Page

All lanes:

Western blot - Anti-ENPP6 antibody (ab127897) at 1/1000 dilution

All lanes:

MCF7 whole cell lysate at 30 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ENPP6 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q6UWR7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENPP6 also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 6 functions as a choline-specific glycerophosphodiester phosphodiesterase. This enzyme has a role in the hydrolysis of choline-containing phosphodiester substrates to produce choline and glycerophosphates. The molecular weight of ENPP6 is approximately 53 kDa. Expression of ENPP6 occurs largely in the brain particularly in oligodendrocytes as well as in other tissues like the liver and kidneys where it executes its enzymatic roles.
Biological function summary

The enzyme facilitates the regulation of phospholipid metabolism by hydrolyzing lysophosphatidylcholine (LPC) and other similar phospholipids. ENPP6 is recognized for its activity against lysophosphatidic acid (LPA) and with roles in membrane lipid remodeling. Although ENPP6 does not form part of a larger complex its action impacts lipid signaling molecules thereby influencing cellular signaling and membrane dynamics.

Pathways

It significantly influences the lipid metabolic pathways and the choline metabolism in cancer (CHOL) pathway. ENPP6 plays a critical role in generating LPA from LPC which is part of the lipid signaling pathway that involves LPA as a signaling molecule. It interacts with proteins like LPAR1 and LPCAT1 which play roles in lipid signaling and remodeling pathways.

The dysregulation of ENPP6 has associations with metabolic and neurodegenerative conditions. Altered levels of ENPP6 activity are observed in Alzheimer's disease where its function may impact neuronal health due to imbalanced phospholipid levels. In cancer ENPP6 overactivity has correlations with cancer cell proliferation within the choline metabolism pathway. It connects to proteins such as APP and WNT5A in disease contexts playing roles in abnormal signaling and cellular alterations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Choline-specific glycerophosphodiesterase that hydrolyzes glycerophosphocholine (GPC) and lysophosphatidylcholine (LPC) and contributes to supplying choline to the cells (PubMed : 15788404). Has a preference for LPC with short (12 : 0 and 14 : 0) or polyunsaturated (18 : 2 and 20 : 4) fatty acids. In vitro, hydrolyzes only choline-containing lysophospholipids, such as sphingosylphosphorylcholine (SPC), platelet-activating factor (PAF) and lysoPAF, but not other lysophospholipids (By similarity).
See full target information ENPP6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC neuroscience 18:63 PubMed28806929

2017

Myelination is delayed during postnatal brain development in the mdx mouse model of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Azeez Aranmolate,Nathaniel Tse,Holly Colognato
View all publications
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Product promise

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