AB224564

Anti-ENPP6 antibody

Name: Anti-ENPP6 antibody (ab224564) | Abcam
Brand: Abcam Limited
SKU: AB224564

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(2 Publications)

Rabbit Polyclonal ENPP6 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP6 aa 1-150.

View Alternative Names

UNQ1889/PRO4334, ENPP6, Glycerophosphocholine cholinephosphodiesterase ENPP6, GPC-Cpde, Choline-specific glycerophosphodiester phosphodiesterase, Ectonucleotide pyrophosphatase/phosphodiesterase family member 6, E-NPP 6, NPP-6

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP6 antibody (AB224564)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP6 antibody (AB224564)

Paraffin-embedded human kidney tissue stained for ENPP6 using ab224564 at 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ENPP6 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q6UWR7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENPP6 also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 6 functions as a choline-specific glycerophosphodiester phosphodiesterase. This enzyme has a role in the hydrolysis of choline-containing phosphodiester substrates to produce choline and glycerophosphates. The molecular weight of ENPP6 is approximately 53 kDa. Expression of ENPP6 occurs largely in the brain particularly in oligodendrocytes as well as in other tissues like the liver and kidneys where it executes its enzymatic roles.

Biological function summary

The enzyme facilitates the regulation of phospholipid metabolism by hydrolyzing lysophosphatidylcholine (LPC) and other similar phospholipids. ENPP6 is recognized for its activity against lysophosphatidic acid (LPA) and with roles in membrane lipid remodeling. Although ENPP6 does not form part of a larger complex its action impacts lipid signaling molecules thereby influencing cellular signaling and membrane dynamics.

Pathways

It significantly influences the lipid metabolic pathways and the choline metabolism in cancer (CHOL) pathway. ENPP6 plays a critical role in generating LPA from LPC which is part of the lipid signaling pathway that involves LPA as a signaling molecule. It interacts with proteins like LPAR1 and LPCAT1 which play roles in lipid signaling and remodeling pathways.

The dysregulation of ENPP6 has associations with metabolic and neurodegenerative conditions. Altered levels of ENPP6 activity are observed in Alzheimer's disease where its function may impact neuronal health due to imbalanced phospholipid levels. In cancer ENPP6 overactivity has correlations with cancer cell proliferation within the choline metabolism pathway. It connects to proteins such as APP and WNT5A in disease contexts playing roles in abnormal signaling and cellular alterations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Choline-specific glycerophosphodiesterase that hydrolyzes glycerophosphocholine (GPC) and lysophosphatidylcholine (LPC) and contributes to supplying choline to the cells (PubMed : 15788404). Has a preference for LPC with short (12 : 0 and 14 : 0) or polyunsaturated (18 : 2 and 20 : 4) fatty acids. In vitro, hydrolyzes only choline-containing lysophospholipids, such as sphingosylphosphorylcholine (SPC), platelet-activating factor (PAF) and lysoPAF, but not other lysophospholipids (By similarity).

See full target information ENPP6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMJ open diabetes research & care 11: PubMed37253485

2023

Fecal microbiota transplantation ameliorates type 2 diabetes via metabolic remodeling of the gut microbiota in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Chen,Lin Guo,Susu Feng,Congcong Wang,Zhicheng Cui,Sijing Wang,Qingmiao Lu,Hang Chang,Bo Hang,Antoine M Snijders,Jian-Hua Mao,Yibing Lu,Dafa Ding

JBMR plus 5:e10439 PubMed33615108

2021

Ablation of Results in Transient Bone Hypomineralization.

Applications

Unspecified application

Species

Unspecified reactive species

Scott Dillon,Karla Suchacki,Shun-Neng Hsu,Louise A Stephen,Rongling Wang,William P Cawthorn,Alan J Stewart,Fabio Nudelman,Nicholas M Morton,Colin Farquharson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENPP6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP6 antibody (AB224564)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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