Rabbit Polyclonal ENPP6 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP6 aa 1-150.
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Publications
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- JBMR plus 5:e104392021Ablation of Results in Transient Bone Hypomineralization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott Dillon,Karla Suchacki,Shun-Neng Hsu,Louise A Stephen,Rongling Wang,William P Cawthorn,Alan J Stewart,Fabio Nudelman,Nicholas M Morton,Colin FarquharsonPubMed 33615108
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ENPP6 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UWR7
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Orangutan | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Choline-specific glycerophosphodiesterase that hydrolyzes glycerophosphocholine (GPC) and lysophosphatidylcholine (LPC) and contributes to supplying choline to the cells (PubMed:15788404). Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. In vitro, hydrolyzes only choline-containing lysophospholipids, such as sphingosylphosphorylcholine (SPC), platelet-activating factor (PAF) and lysoPAF, but not other lysophospholipids (By similarity).
Alternative names
UNQ1889/PRO4334, ENPP6, Glycerophosphocholine cholinephosphodiesterase ENPP6, GPC-Cpde, Choline-specific glycerophosphodiester phosphodiesterase, Ectonucleotide pyrophosphatase/phosphodiesterase family member 6, E-NPP 6, NPP-6
Recommended products
Rabbit Polyclonal ENPP6 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ENPP6 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ENPP6 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UWR7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ENPP6 also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 6 functions as a choline-specific glycerophosphodiester phosphodiesterase. This enzyme has a role in the hydrolysis of choline-containing phosphodiester substrates to produce choline and glycerophosphates. The molecular weight of ENPP6 is approximately 53 kDa. Expression of ENPP6 occurs largely in the brain particularly in oligodendrocytes as well as in other tissues like the liver and kidneys where it executes its enzymatic roles.
- Biological function summary
The enzyme facilitates the regulation of phospholipid metabolism by hydrolyzing lysophosphatidylcholine (LPC) and other similar phospholipids. ENPP6 is recognized for its activity against lysophosphatidic acid (LPA) and with roles in membrane lipid remodeling. Although ENPP6 does not form part of a larger complex its action impacts lipid signaling molecules thereby influencing cellular signaling and membrane dynamics.
- Pathways
It significantly influences the lipid metabolic pathways and the choline metabolism in cancer (CHOL) pathway. ENPP6 plays a critical role in generating LPA from LPC which is part of the lipid signaling pathway that involves LPA as a signaling molecule. It interacts with proteins like LPAR1 and LPCAT1 which play roles in lipid signaling and remodeling pathways.
- Associated diseases and disorders
The dysregulation of ENPP6 has associations with metabolic and neurodegenerative conditions. Altered levels of ENPP6 activity are observed in Alzheimer's disease where its function may impact neuronal health due to imbalanced phospholipid levels. In cancer ENPP6 overactivity has correlations with cancer cell proliferation within the choline metabolism pathway. It connects to proteins such as APP and WNT5A in disease contexts playing roles in abnormal signaling and cellular alterations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP6 antibody (ab224564)
Paraffin-embedded human kidney tissue stained for ENPP6 using ab224564 at 1/20 dilution in immunohistochemical analysis.
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