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AB125873

Anti-ENSA antibody

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(1 Publication)

Rabbit Polyclonal ENSA antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Alpha-endosulfine aa 1 to C-terminus.

View Alternative Names

Alpha-endosulfine, ARPP-19e, ENSA

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ENSA antibody (AB125873)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ENSA antibody (AB125873)

Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ENSA (green) using ab125873 at 1/500 dilution in ICC/IF. Blue : Hoechst 33343 staining.

Western blot - Anti-ENSA antibody (AB125873)
  • WB

Lab

Western blot - Anti-ENSA antibody (AB125873)

All lanes:

Western blot - Anti-ENSA antibody (ab125873) at 1/500 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

ENSA Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Alpha-endosulfine aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43768

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENSA also known as alpha-endosulfine is a protein with a mass of approximately 13 kDa. ENSA is part of the endosulfine family and finds expression in various tissues including the brain and pancreas. This protein primarily acts as an endogenous ligand for sulfonylurea receptor and has a role in regulating potassium channels. Its structural and functional characteristics highlight its importance in cellular activities.
Biological function summary

ENSA functions as a modulator of protein phosphatase 2A (PP2A) a critical enzyme in controlling cell growth and division. ENSA by inhibiting the catalytic activity of PP2A influences key regulatory mechanisms in cells. It also interacts with ARPP-19 a protein that enhances its inhibitory action contributing to the regulation of cellular processes such as mitosis and signal transduction.

Pathways

ENSA plays a significant role in the mTOR signaling pathway a major regulator of cell growth metabolism and survival. In this pathway ENSA interacts with various proteins including the mTOR itself to modulate the phosphorylation state of downstream targets affecting cellular responses to nutrient availability. Additionally ENSA is involved in the regulation of glucose metabolism through its interaction in the AMPK signaling pathway contributing to energy homeostasis in cells.

ENSA demonstrates a connection to metabolic disorders such as type 2 diabetes by modulating insulin secretion and action. ENSA impacts the glucose regulatory processes through its association with proteins like the sulfonylurea receptor important in insulin release from pancreatic beta cells. Moreover ENSA's involvement in the dysregulation of cell growth pathways relates to certain cancers indicating its potential role in tumorigenesis through its interaction with PP2A and associated signaling components.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
See full target information Alpha-endosulfine
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37432316

2023

Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset.

Applications

Unspecified application

Species

Unspecified reactive species

Arian Mansur,Remi Joseph,Euri S Kim,Pierre M Jean-Beltran,Namrata D Udeshi,Cadence Pearce,Hanjie Jiang,Reina Iwase,Miroslav P Milev,Hashem A Almousa,Elyshia McNamara,Jeffrey Widrick,Claudio Perez,Gianina Ravenscroft,Michael Sacher,Philip A Cole,Steven A Carr,Vandana A Gupta
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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