AB180513

Anti-ENSA antibody [EPR8008(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal ENSA antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

Alpha-endosulfine, ARPP-19e, ENSA

3 Images

Flow Cytometry (Intracellular) - Anti-ENSA antibody [EPR8008(2)] (AB180513)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ENSA antibody [EPR8008(2)] (AB180513)

Intracellular flow cytometric analysis of K562 cells labeling ENSAwith ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde.

Supplier Data

Western blot - Anti-ENSA antibody [EPR8008(2)] (AB180513)

Blocking/diuting buffer buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/2000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody at 1/1000 dilution

Predicted band size: 13 kDa

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Supplier Data

Western blot - Anti-ENSA antibody [EPR8008(2)] (AB180513)

Blocking/dilutin buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/10000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8008(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ENSA (O43768) and ARPP-19 (P56211)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/70", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENSA also known as alpha-endosulfine is a protein with a mass of approximately 13 kDa. ENSA is part of the endosulfine family and finds expression in various tissues including the brain and pancreas. This protein primarily acts as an endogenous ligand for sulfonylurea receptor and has a role in regulating potassium channels. Its structural and functional characteristics highlight its importance in cellular activities.

Biological function summary

ENSA functions as a modulator of protein phosphatase 2A (PP2A) a critical enzyme in controlling cell growth and division. ENSA by inhibiting the catalytic activity of PP2A influences key regulatory mechanisms in cells. It also interacts with ARPP-19 a protein that enhances its inhibitory action contributing to the regulation of cellular processes such as mitosis and signal transduction.

Pathways

ENSA plays a significant role in the mTOR signaling pathway a major regulator of cell growth metabolism and survival. In this pathway ENSA interacts with various proteins including the mTOR itself to modulate the phosphorylation state of downstream targets affecting cellular responses to nutrient availability. Additionally ENSA is involved in the regulation of glucose metabolism through its interaction in the AMPK signaling pathway contributing to energy homeostasis in cells.

ENSA demonstrates a connection to metabolic disorders such as type 2 diabetes by modulating insulin secretion and action. ENSA impacts the glucose regulatory processes through its association with proteins like the sulfonylurea receptor important in insulin release from pancreatic beta cells. Moreover ENSA's involvement in the dysregulation of cell growth pathways relates to certain cancers indicating its potential role in tumorigenesis through its interaction with PP2A and associated signaling components.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

See full target information Alpha-endosulfine

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8016 PubMed40866357

2025

The balance between B55α and Greatwall expression levels predicts sensitivity to Greatwall inhibition in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Róbert Zach,Michael Annis,Sandra M Martin-Guerrero,Abdulrahman Alatawi,Kim Hou Chia,Megan Meredith,Kay Osborn,Nisha Peter,William Pearce,Jessica Booth,Mohan Rajasekaran,Samantha Dias,Lily Coleman-Evans,William R Foster,Jon A Harper,Alex D Herbert,Catherine Tighe,Tristan Reuillon,Ryan West,Oliver Busby,Kamila Burdova,Damien Crepin,Sergi Ortoll,Kulthida Vaeteewoottacharn,Donniphat Dejsuphong,John Spencer,Hitesh Patel,Darren Le Grand,Thomas A Hunt,David M Andrews,Hiroyuki Yamano,Pedro R Cutillas,Antony W Oliver,Simon E Ward,Helfrid Hochegger

Science advances 10:eadh9547 PubMed38489372

2024

CKLF instigates a "cold" microenvironment to promote MYCN-mediated tumor aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodan Qin,Andrew Lam,Xu Zhang,Satyaki Sengupta,J Bryan Iorgulescu,Hongru Ni,Sanjukta Das,Madison Rager,Zhenwei Zhou,Tao Zuo,Grace K Meara,Alexander E Floru,Chinyere Kemet,Divya Veerapaneni,Daniel Kashy,Liang Lin,Kenneth Lloyd,Lauren Kwok,Kaylee S Smith,Raghavendar T Nagaraju,Rob Meijers,Craig Ceol,Ching-Ti Liu,Sanda Alexandrescu,Catherine J Wu,Derin B Keskin,Rani E George,Hui Feng

Frontiers in cell and developmental biology 10:904719 PubMed36247015

2022

The MASTL-ENSA-PP2A/B55 axis modulates cisplatin resistance in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Odjo G Gouttia,Jing Zhao,Yanqiu Li,Mackenzie J Zwiener,Ling Wang,Gregory G Oakley,Aimin Peng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENSA antibody [EPR8008(2)]

Flow Cytometry (Intracellular) - Anti-ENSA antibody [EPR8008(2)] (AB180513)

Western blot - Anti-ENSA antibody [EPR8008(2)] (AB180513)

Western blot - Anti-ENSA antibody [EPR8008(2)] (AB180513)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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