AB190877

Anti-ENT1 antibody - N-terminal

Name: Anti-ENT1 antibody - N-terminal (ab190877) | Abcam
Brand: Abcam Limited
SKU: AB190877
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(1 Publication)

Goat Polyclonal ENT1 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC29A1 aa 1-50.

View Alternative Names

ENT1, SLC29A1, Equilibrative nucleoside transporter 1, hENT1, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Solute carrier family 29 member 1, Equilibrative NBMPR-sensitive nucleoside transporter, es nucleoside transporter

1 Images

Supplier Data

Western blot - Anti-ENT1 antibody - N-terminal (AB190877)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-ENT1 antibody - N-terminal (ab190877) at 1 µg/mL

Lane 1:

Human breast tissue lysates at 35 µg

Lane 2:

Human placenta tissue lysates at 35 µg

Lane 3:

Human spleen tissue lysates at 35 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SLC29A1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q99808

Specificity

Reported variants represent identical protein: NP_001071642.1, NP_001071645.1, NP_001071644.1, NP_004946.1, NP_001071643.1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab190877 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENT1 also known as Equilibrative Nucleoside Transporter 1 or SLC29A1 is a membrane protein responsible for transporting nucleosides across cell membranes. This protein weighs approximately 55 kDa and is expressed in various tissues including the brain heart and liver. The transport activity of ENT1 plays a fundamental role in maintaining cellular nucleoside levels. The efficient transport mechanisms of ENT1 highlight its significance in regulating the availability of substrates for nucleic acid synthesis.

Biological function summary

This transporter facilitates the uptake and recycling of nucleosides which are essential building blocks for DNA and RNA synthesis. ENT1 mediates the transport of adenosine an important nucleoside involved in multiple cellular functions. It operates as a component of a broader transport system that includes similar proteins like ENT2. This action contributes to cellular processes by controlling nucleoside concentrations impacting cell proliferation and metabolic activities.

Pathways

ENT1 plays a role in the purine salvage pathway and adenosine signaling. It regulates extracellular adenosine levels influencing adenosine receptor-mediated pathways that impact many physiological functions. ENT1 coordinates with proteins such as adenosine kinases to manage the intracellular balance of adenosine therefore integrating into both metabolic and signaling routes. Its role in these pathways affects energy metabolism and supports cellular responses to stress.

ENT1 has been associated with conditions such as cardiovascular diseases and neurological disorders. Altered ENT1 activity can affect adenosine levels leading to variations in blood flow and neurotransmission. In cardiovascular diseases ENT1 modulates adenosine's cardioprotective effects. In neurological contexts dysfunction of ENT1 relates to disorders involving adenosine receptor signaling where its interaction with proteins like ADA (adenosine deaminase) can influence disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed : 10722669, PubMed : 10755314, PubMed : 12527552, PubMed : 14759222, PubMed : 15037197, PubMed : 17379602, PubMed : 21795683, PubMed : 26406980, PubMed : 27995448, PubMed : 35790189, PubMed : 8986748). Functions as a Na(+)-independent transporter (PubMed : 8986748). Involved in the transport of nucleosides such as adenosine, guanosine, inosine, uridine, thymidine and cytidine (PubMed : 10722669, PubMed : 10755314, PubMed : 12527552, PubMed : 14759222, PubMed : 15037197, PubMed : 17379602, PubMed : 26406980, PubMed : 8986748). Also transports purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed : 21795683, PubMed : 27995448). Mediates basolateral nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis barrier (By similarity). Regulates inosine levels in brown adipocytes tissues (BAT) and extracellular inosine levels, which controls BAT-dependent energy expenditure (PubMed : 35790189).

See full target information SLC29A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 4:775 PubMed34163008

2021

Cyanocobalamin prevents cardiomyopathy in type 1 diabetes by modulating oxidative stress and DNMT-SOCS1/3-IGF-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Masao Kakoki,Purushotham V Ramanathan,John R Hagaman,Ruriko Grant,Jennifer C Wilder,Joan M Taylor,J Charles Jennette,Oliver Smithies,Nobuyo Maeda-Smithies

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENT1 antibody - N-terminal

Western blot - Anti-ENT1 antibody - N-terminal (AB190877)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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