AB272278

Anti-Entactin/NID antibody [EPR22414-114] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Entactin/NID antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Ent, Nid1, Nidogen-1, NID-1, Entactin

1 Images

Sandwich ELISA - Anti-Entactin/NID antibody [EPR22414-114] - BSA and Azide free (Detector) (AB272278)

sELISA

Supplier Data

Sandwich ELISA - Anti-Entactin/NID antibody [EPR22414-114] - BSA and Azide free (Detector) (AB272278)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270203).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22414-114

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab272278 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Nidogen-1. The recommended pair for sandwich ELISA is:
Capture: ab272277, Mouse Nidogen-1 Capture Antibody (unconjugated)
Detector: ab272278, Mouse Nidogen-1 Detector Antibody (unconjugated)
The reference range value is 78.125 - 5000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.

Biological function summary

Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.

Pathways

Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.

Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

See full target information Nid1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Entactin/NID antibody [EPR22414-114] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-Entactin/NID antibody [EPR22414-114] - BSA and Azide free (Detector) (AB272278)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise