Rabbit Recombinant Monoclonal Entactin/NID antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89743.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81772.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325)")
![Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-frozen-sections-img108575.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325)")
![Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-frozen-sections-img99004.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (AB254325)")
![Western blot - Anti-Entactin/NID antibody [EPR22414-125] (AB254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--western-blot-img16013.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Entactin/NID antibody [EPR22414-125] (AB254325)")
Publications
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- Science advances 9:eade25142023Engineering edgeless human skin with enhanced biomechanical properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Pappalardo,David Alvarez Cespedes,Shuyang Fang,Abigail R Herschman,Eun Young Jeon,Kristin M Myers,Jeffrey W Kysar,Hasan Erbil AbaciPubMed 36706190
- International journal of molecular sciences 21:2019Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSekyoo Jeong et. al.PubMed 31861912
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Expected |
| Mouse | Not recommended | Tested | Tested | Tested |
| Rat | Not recommended | Tested | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Species Rat | Dilution info 1/500 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes IHC recommended for Mouse and Rat only. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes IHC recommended for Mouse and Rat only. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Alternative names
NID, NID1, Nidogen-1, NID-1, Entactin
Recommended products
Rabbit Recombinant Monoclonal Entactin/NID antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22414-125
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.
- Biological function summary
Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.
- Pathways
Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.
- Associated diseases and disorders
Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89743.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on basement membrane of rat cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81772.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on basement membrane of mouse cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-frozen-sections-img108575.jpg "Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
![Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--immunohistochemistry-frozen-sections-img99004.jpg "Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
Immunohistochemical analysis of 4% Paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
![Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--western-blot-img16013.jpg "Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
Blocking/diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:3084254,12215539, 22952693).
All lanes: Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325) at 1/1000 dilution
Lane 1: Mouse heart tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate at 20 µg
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Lane 5: Rat lung tissue lysate at 20 µg
Lane 6: Rat skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 136 kDa
Exposure time: 3s
![Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--western-blot-img6092.jpg "Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
Blocking/diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:3084254, 12215539, 22952693).
All lanes: Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
Lane 3: Human placenta tissue lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 136 kDa
Observed band size: 100 kDa, 160 kDa
Exposure time: 8s
![Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--western-blot-img98685.jpg "Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325) at 1/1000 dilution
All lanes: Recombinant human Entactin/NID protein (aa29 -1114) 20 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 136 kDa
Observed band size: 130 kDa
![Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--western-blot-img6092.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
![Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325), expandable thumbnail](https://content.abcam.com/products/images/entactin-nid-antibody-epr22414-125-ab254325--western-blot-img98685.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)")
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