Anti-entB antibody

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EntB protein also known as 23-dihydroxybenzoate-AMP ligase is essential for the biosynthesis of enterobactin the siderophore responsible for iron transport in bacteria. This protein has a molecular mass of approximately 60 kDa and is expressed predominantly in Escherichia coli. EntB plays a mechanical role as an isochorismate lyase converting isochorismate to 23-dihydroxybenzoate (23-DHB) which is a precursor in enterobactin synthesis. This step is important for high-affinity iron uptake especially under iron-limiting conditions allowing bacteria to thrive in diverse environments.

Biological function summary

EntB is involved in the enterobactin biosynthesis pathway which plays a pivotal role in iron acquisition by pathogenic bacteria helping in evading host immune responses. EntB functions as a part of the enterobactin synthetase multi-enzyme complex working synergistically with other proteins like EntD EntE and EntF. Within this complex EntB contributes to the assembly of enterobactin helping the bacteria secure necessary iron from the host environment or surroundings which supports bacterial growth and survival.

Pathways

EntB's function integrates into the non-ribosomal peptide synthetase (NRPS) system for siderophore biosynthesis intersecting with the shikimate pathway. EntB's activity links to enzymes like EntC and EntH playing a part in converting chorismate to enterobactin. Additionally the shikimate pathway serves as a precursor for aromatic amino acids whose role extends beyond siderophore production integrating into broader bacterial metabolic networks.

EntB's role in enterobactin production links it to bacterial virulence and pathogenicity. Pathogenic strains of bacteria like E. coli and Salmonella use EntB for iron acquisition making it important for infection processes including urinary tract infections and systemic infections. By interacting with proteins like EntA and other components of the enterobactin biosynthetic pathway EntB becomes a potential target for antimicrobial drug development aiming to disrupt the bacterial siderophore-mediated iron acquisition thereby attenuating the bacterial virulence and reducing infection rates.