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AB200691

Anti-Enterokinase antibody

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(1 Publication)

Rabbit Polyclonal Enterokinase antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TMPRSS15 aa 750-850.

View Alternative Names

ENTK, PRSS7, TMPRSS15, Enteropeptidase, Enterokinase, Serine protease 7, Transmembrane protease serine 15

1 Images
Western blot - Anti-Enterokinase antibody (AB200691)
  • WB

Supplier Data

Western blot - Anti-Enterokinase antibody (AB200691)

All lanes:

Western blot - Anti-Enterokinase antibody (ab200691)

Lane 1:

A549 whole cell lysate

Lane 2:

Raw264.7 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 113 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TMPRSS15 aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

P98073

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Enterokinase also known as enteropeptidase is a serine protease enzyme with a significant role in the digestive process. It exhibits a mass of approximately 150 kDa and predominantly localizes in the brush border of the small intestine particularly in the duodenum. Enterokinase activates trypsinogen to trypsin by cleaving a specific lysine-isoleucine peptide bond which is a critical step in protein digestion. This enzyme exists as a membrane-bound protein facilitating its interaction with substrates within the intestinal lumen.
Biological function summary

Enterokinase triggers the activation cascade of pancreatic serine proteases necessary for protein breakdown. It acts as a gateway enzyme for the activation of several inactive enzymes into their active forms. Enterokinase functions independently and is not part of a large protein complex. Its role ensures efficient conversion of digestive enzymes like trypsinogen highlighting its importance in nutrient assimilation.

Pathways

Enterokinase plays a role in the digestive enzyme activation pathway specifically initiating the conversion of key proenzymes into active forms. The enzyme is integral to the protein digestion pathway along with other proteases like trypsin and chymotrypsin which further break down proteins into peptides and amino acids. Enterokinase's activity is essential for the downstream function of these proteases emphasizing its place at the pathway's front line.

Enterokinase deficiency can lead to severe protein malnutrition as seen in enterokinase deficiency disorder which results in an inability to fully digest dietary proteins. Additionally alterations in enterokinase function may relate to digestive disturbances and pancreatic disorders. This relationship underlines the enzyme's connection with trypsin heightened in cases where trypsinogen conversion is impaired further affecting digestive efficiency.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
See full target information TMPRSS15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

RNA biology 17:784-793 PubMed32174258

2020

LncRNA MAGI2-AS3 inhibits the self-renewal of leukaemic stem cells by promoting TET2-dependent DNA demethylation of the LRIG1 promoter in acute myeloid leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Chen,Xu Fan,Jianhua Zhu,Xuexin Chen,Yiling Liu,Hao Zhou
View all publications
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