Anti-Enterovirus 71 VP1 antibody [HL1929]

5 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-Enterovirus 71 VP1 antibody [HL1929] (ab308206)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed Mock and transfected 293T cells labelling Enterovirus 71 VP1 with ab308206 at 1/500 dilution at RT for 15 min.

  Blue: Fluoroshield with DAPI.

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  Western blot - Anti-Enterovirus 71 VP1 antibody [HL1929] (ab308206)

  Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (ab308206) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  Lane 1: Non-transfected 293T whole cell extracts at 30 µg

  Lane 2: Enterovirus-transfected (+) 293T whole cell extracts at 30 µg

  Secondary

  All lanes: HRP-conjugated anti-rabbit IgG antibody

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  Western blot - Anti-Enterovirus 71 VP1 antibody [HL1929] (ab308206)

  Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (ab308206) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  Lane 1: Non-infected RD whole cell extracts at 20 µg

  Lane 2: Enterovirus 71 C2 infected RD whole cell extracts at 20 µg

  Lane 3: Enterovirus D68 infected RD whole cell extracts at 20 µg

  Lane 4: Coxsackievirus A6 infected RD whole cell extracts at 20 µg

  Secondary

  All lanes: HRP-conjugated anti-rabbit IgG antibody

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  Western blot - Anti-Enterovirus 71 VP1 antibody [HL1929] (ab308206)

  Non-infected (–) and infected (+) RD whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (ab308206) diluted at 1:1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  Lane 1: Non-infected (–) RD whole cell extracts at 5 µg

  Lane 2: Infected (+) RD whole cell extracts at 5 µg

  Secondary

  All lanes: HRP-conjugated anti-rabbit IgG antibody

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  Indirect ELISA - Anti-Enterovirus 71 VP1 antibody [HL1929] (ab308206)

  Indirect ELISA analysis was performed by coating the plate with recombinant Enterovirus 71 VP1 protein, His tag (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [HL1929] (ab308206) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.