Anti-Enterovirus 71 VP1 antibody [HL1929]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Enterovirus 71 VP1 antibody [HL1929] (AB308206)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Mock and transfected 293T cells labelling Enterovirus 71 VP1 with ab308206 at 1/500 dilution at RT for 15 min.

Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-Enterovirus 71 VP1 antibody [HL1929] (AB308206)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (ab308206) diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Enterovirus-transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-Enterovirus 71 VP1 antibody [HL1929] (AB308206)

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (ab308206) diluted at 1 : 20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-infected RD whole cell extracts at 20 µg

Lane 2:

Enterovirus 71 C2 infected RD whole cell extracts at 20 µg

Lane 3:

Enterovirus D68 infected RD whole cell extracts at 20 µg

Lane 4:

Coxsackievirus A6 infected RD whole cell extracts at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-Enterovirus 71 VP1 antibody [HL1929] (AB308206)

Non-infected (–) and infected (+) RD whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [HL1929] (ab308206) diluted at 1 : 1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-infected (–) RD whole cell extracts at 5 µg

Lane 2:

Infected (+) RD whole cell extracts at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Enterovirus 71 VP1 antibody [HL1929] (AB308206)

Indirect ELISA analysis was performed by coating the plate with recombinant Enterovirus 71 VP1 protein, His tag (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [HL1929] (ab308206) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (1 : 10000) was used to detect the bound primary antibody.