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AB308217

Anti-Enterovirus D68 VP1 antibody [HL1997]

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Rabbit Recombinant Monoclonal Enterovirus D68 VP1 antibody antibody. Carrier free. Suitable for sELISA, WB and reacts with Recombinant full length protein - Enterovirus D68, Enterovirus D68, Transfected cell lysate - Enterovirus D68 samples.
5 Images
Western blot - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)
  • WB

Supplier Data

Western blot - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (ab308217) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-infected RD whole cell extracts at 30 µg

Lane 2:

Infected RD whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)
  • WB

Supplier Data

Western blot - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (ab308217) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-transfected 293T cell extracts at 30 µg

Lane 2:

DDDDK- tagged enterovirus D68 VP1 transfected 293T whole cell extracts at 30 µg

Lane 3:

DDDDK- tagged enterovirus 71 VP1 transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)
  • WB

Supplier Data

Western blot - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (ab308217) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-infected RD whole cell extracts at 20 µg

Lane 2:

Enterovirus 71 C2 infected RD whole cell extracts at 20 µg

Lane 3:

Enterovirus D68 infected RD whole cell extracts at 20 µg

Lane 4:

Coxsackievirus A6 infected RD whole cell extracts at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Sandwich ELISA - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)

Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (D68; 71) using ab308217 as detection antibody (1 μg/mL).

Sandwich ELISA - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Enterovirus D68 VP1 antibody [HL1997] (AB308217)

Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (D68; 71) using antibodies as below : Capture : ab308408 (5 μg/mL) Detection : ab308217 (1 μg/mL)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1997

Isotype

IgG

Carrier free

Yes

Reacts with

Enterovirus D68

Applications

WB, sELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Enterovirus D68": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Enterovirus D68": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Please notice that ab308217 needs to be conjugated to HRP to function as the detection antibody when paired with <a href='/en-us/products/primary-antibodies/enterovirus-d68-vp1-antibody-gt1843-bsa-and-azide-free-ab308408'>ab308408</a>.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Enterovirus D68": { "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Enterovirus VP1 also known as viral protein 1 is a structural protein of the enterovirus capsid. It has a mass of approximately 30 kilodaltons and plays an important role in virus assembly and host cell interaction. The protein is expressed on the viral surface and it is responsible for initial binding to the host cell receptors. This interaction facilitates the entry of the virus into cells initiating the infectious cycle. Enterovirus VP1 is highly conserved among different enterovirus serotypes which allows it to serve as a target for diagnostics and therapeutic interventions.
Biological function summary

This protein enables the structural integrity and infectivity of the virus. Enterovirus VP1 is a part of the viral capsid a protein complex that also includes VP2 VP3 and VP4. These proteins collectively protect the viral RNA genome and are involved in mediating interactions with the host immune system. The conformational structure of VP1 is recognized by neutralizing antibodies making it a focus point for vaccine development and antiviral drugs. Its expression and conformation can influence the antigenicity and serotype specificity of enteroviruses.

Pathways

Several actions revolving around host-pathogen interactions involve Enterovirus VP1. It plays a pivotal role in the cell entry mechanism through endocytic pathways. VP1 interacts with proteins such as ICAM-1 or PSGL-1 which serves as receptors in the early stages of viral pathogenesis. Additionally during viral uncoating and RNA release VP1 undergoes structural rearrangements that are essential for subsequent steps in the viral lifecycle and involve complex pathways like the host cell defense mechanism.

Alterations in Enterovirus VP1 are significant in the context of ailments like hand foot and mouth disease and viral myocarditis. The protein's sequence variations can influence disease severity and transmission. Furthermore correlations have been established between the structural aspects of VP1 and host proteins involved in disease manifestation such as cellular kinases influencing viral replication. This protein's interactions and mutations contribute to the pathogenicity and epidemiology of enterovirus-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Enterovirus D68 VP1 antibody
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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