JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247934

Anti-ENTPD2 antibody [EPR3885] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal ENTPD2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD39L1, ENTPD2, Ectonucleoside triphosphate diphosphohydrolase 2, E-NTPDase 2, NTPDase 2, CD39 antigen-like 1, Ecto-ATP diphosphohydrolase 2, Ecto-ATPDase 2, Ecto-ATPase 2

1 Images
Western blot - Anti-ENTPD2 antibody [EPR3885] - BSA and Azide free (AB247934)
  • WB

Unknown

Western blot - Anti-ENTPD2 antibody [EPR3885] - BSA and Azide free (AB247934)

This data was developed using ab110711, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ENTPD2 antibody [EPR3885] - BSA and Azide free (ab247934) at 1/1000 dilution

Lane 1:

Human placenta cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

Human fetal kidney cell lysate at 10 µg

Lane 4:

U-87MG cell lysate at 10 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3885

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB51601

Anti-DAPK2 antibody [EP1633Y]

RabMAb

Flow Cytometry (Intracellular) - Anti-DAPK2 antibody [EP1633Y] (AB51601)

  • 4
  • 1
Primary Antibodies

AB247930

Anti-Cortisol Binding Globulin antibody [EPR4615] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Cortisol Binding Globulin antibody [EPR4615] - BSA and Azide free (AB247930)

  • 0
  • 0
Primary Antibodies

AB108444

Anti-P2Y13 antibody [EPR3698]

RabMAb|Recombinant

Western blot - Anti-P2Y13 antibody [EPR3698] (AB108444)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab247934 is the carrier-free version of ab110711.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENTPD2 also known as ectonucleoside triphosphate diphosphohydrolase 2 or CD39L1 functions as an enzyme that hydrolyzes nucleoside triphosphates and diphosphates into their respective monophosphates while releasing inorganic phosphate. It has a molecular mass of approximately 61 kDa. This protein is expressed in many tissues with a notable presence in the human liver placenta and pancreas. Its expression on endothelial and epithelial cells suggests active involvement in vascular and surface barrier systems.
Biological function summary

ENTPD2 participates in the regulation of extracellular nucleotide levels which influences various physiological processes. It works as part of the nucleoside triphosphate diphosphohydrolase family that controls purinergic signaling by degrading high-energy nucleotides. This regulation affects cellular activities such as proliferation and apoptosis. Its role is critical in modulating the availability of nucleotides for homeostatic and stress responses in the body.

Pathways

ENTPD2 is involved in purinergic signaling and ATP metabolism pathways. These pathways are significant in maintaining cellular energy balance and facilitating communication between cells through signaling molecules like ATP and ADP. ENTPD2 has a functional interplay with other proteins like ENTPD1 and adenosine deaminase during these processes ensuring tight regulation of extracellular nucleotides and their products.

ENTPD2 shows associations with cancer particularly hepatocellular carcinoma and inflammatory diseases. It is an important player in cancer due to its ability to modulate the tumor microenvironment through purinergic signaling influencing tumor growth and metastasis. Inflammatory responses also connect to ENTPD2 activity where altered expression can contribute to chronic inflammation conditions. The protein's interaction with CD39 another member of the ectonucleotidase family highlights its important role in these pathological contexts.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP.
See full target information ENTPD2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 136:2003-2012 PubMed27349864

2016

Thyroid Hormones Enhance Mitochondrial Function in Human Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Vidali,Jérémy Chéret,Melanie Giesen,Swantje Haeger,Majid Alam,Rachel E B Watson,Abigail K Langton,Matthias Klinger,Jana Knuever,Wolfgang Funk,Barbara Kofler,Ralf Paus
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com