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AB317148

Anti-Eosinophil derived neurotoxin antibody [HL2166]

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Rabbit Monoclonal Eosinophil derived neurotoxin antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RNASE2.

View Alternative Names

EDN, RNS2, RNASE2, Non-secretory ribonuclease, Eosinophil-derived neurotoxin, RNase UpI-2, Ribonuclease 2, Ribonuclease US, RNase 2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eosinophil derived neurotoxin antibody [HL2166] (AB317148)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eosinophil derived neurotoxin antibody [HL2166] (AB317148)

ab317148 detects Eosinophil derived neurotoxin protein at cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded human kidney. Eosinophil derived neurotoxin stained by ab317148 diluted at 1 : 200. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-Eosinophil derived neurotoxin antibody [HL2166] (AB317148)
  • WB

Supplier Data

Western blot - Anti-Eosinophil derived neurotoxin antibody [HL2166] (AB317148)

Untreated (–) and treated (+) THP-1 whole cell extract (30 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317148 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Eosinophil derived neurotoxin antibody [HL2166] (ab317148) at 1/1000 dilution

Lane 1:

Untreated (–) HP-1 whole cell extract at 30 µg

Lane 2:

PNGase F, 2hr treated (+) THP-1 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Eosinophil derived neurotoxin antibody [HL2166] (AB317148)
  • WB

Supplier Data

Western blot - Anti-Eosinophil derived neurotoxin antibody [HL2166] (AB317148)

Untreated (–) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 15% SDS-PAGE and the membrane was blotted with ab317148 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Eosinophil derived neurotoxin antibody [HL2166] (ab317148) at 1/1000 dilution

Lane 1:

Untreated (–) THP-1 whole cell extracts at 30 µg

Lane 2:

24 ug/mL Tunicamycin, 16 hr treated (+) THP-1 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2166

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RNASE2.

P10153

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease A also known as RNase A is a type of ribonuclease enzyme known for its ability to cleave RNA molecules. This enzyme specifically catalyzes the endonucleolytic cleavage of RNA to produce 3'-phosphates and 3'-phosphooligonucleotides terminated by cytidine or uridine. RNase A has a molecular mass of approximately 13.7 kDa and is primarily found in the pancreas of bovines. This protein plays a significant role in RNA metabolism and is often utilized in laboratory research for this purpose.
Biological function summary

Ribonuclease A plays an important role in RNA processing and degradation contributing to the regulation of cellular RNA levels. This enzyme does not operate as part of a larger complex but acts independently to degrade single-stranded RNA. The function of RNase A is essential for maintaining cellular homeostasis by controlling the quantity and quality of the cellular RNA ensuring proper turnover and preventing the accumulation of defective or unnecessary RNA molecules.

Pathways

Ribonuclease A is involved in the RNA degradation pathway which is critical for normal cellular function and gene expression regulation. It collaborates with other nucleases to ensure efficient RNA turnover. RNase A is closely related to proteins like RNase 1 and RNase 5 which also participate in RNA metabolism. Participation of RNase A in this pathway highlights its importance in the regulation of genetic information flow from DNA to RNA.

The malfunction or dysregulation of Ribonuclease A can impact diseases such as cancer and cystic fibrosis. In cancer aberrant RNase A activity can influence RNA levels contributing to unchecked cell proliferation. Moreover RNase A is connected to the inflammation seen in cystic fibrosis via its impact on RNA turnover and immune system signaling. RNase A interactions with proteins such as angiogenin which displays similar enzymatic properties further exemplify its involvement in disease-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.
See full target information RNASE2
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Product promise

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