AB133604

Anti-Eotaxin antibody [EPR5825]

20ul selling size
RabMAb
Recombinant
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(8 Publications )

Rabbit Recombinant Monoclonal Eotaxin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (AB133604)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (AB133604)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Eotaxin with unpurified ab133604 at 1/1000.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (AB133604)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Eotaxin with Purified ab133604 at 1 : 100 dilution (1.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

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Western blot - Anti-Eotaxin antibody [EPR5825] (AB133604)

All lanes:

Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/1000 dilution

All lanes:

Eotaxin recombinant protein at 0.01 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 11 kDa

Observed band size: 8 kDa

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Lab

Western blot - Anti-Eotaxin antibody [EPR5825] (AB133604)

Blocking and diluting buffer : 5% NFDM/TBST

Recombinant protein source : Peprotech Inc.
The region it covers : aa24-aa97(8.3kDa)
Tag info : N/A

All lanes:

Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/10000 dilution

All lanes:

Human Eotaxin recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 8 kDa

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OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (AB133604)

OI-RD Scanning

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OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (AB133604)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Eotaxin antibody [EPR5825] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5825

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Note: tested against Eotaxin recombinant protein" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.

Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells (PubMed : 8597956, PubMed : 33846499). Exerts its effects primarily by binding to the CCR3 receptor (PubMed : 8631813). Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway (PubMed : 28279120).

See full target information CCL11

Alternative Names

SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

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Publications (8)

Recent publications for all applications. Explore the full listand refine your search

Cell death discovery 10:294 PubMed38906863

2024

CCL11 released by GSDMD-mediated macrophage pyroptosis regulates angiogenesis after hindlimb ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwen Wang,Yang Gao,Huairui Shi,Rifeng Gao,Ji'e Yang,Ya'nan Qu,Shiyu Hu,Jian Zhang,Jingpu Wang,Jiatian Cao,Feng Zhang,Junbo Ge

Acta dermato-venereologica 104:adv35401 PubMed38348729

2024

Lesional Infiltration of Eosinophils, Basophils, and M2 Macrophages Expressing Eotaxin-1 and Eotaxin-3 in Granuloma Faciale, but not in Erythema Elevatum Diutinum.

Applications

Unspecified application

Species

Unspecified reactive species

Nozomi Akai,Takashi Hashimoto,Manami Okuzawa,Satoshi Okuno,Takahiro Satoh

Critical reviews in eukaryotic gene expression 33:1-12 PubMed37183942

2023

MicroRNA-485-3p Promotes the Inflammatory Response and Extracellular Matrix Deposition by Activating Wnt/β-Catenin Signaling in Human Airway Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiyun Liu,Sen Shi,Ying Gao,Qian Leng,Rui Gong,Lan Zhang,Jinhai Ma

The Laryngoscope 132:2132-2138 PubMed35199865

2022

Primary Hyper-IgE-Related Salivary Gland Disease: A New Disease Entity.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Xuan Zhu,Yan-Yan Zhang,Yan Gao,Yan Chen,Zu-Yan Zhang,Hong Hua,Guang-Yan Yu

The Laryngoscope 131:E800-E806 PubMed32621541

2020

Eosinophilic Sialodochitis: A Type of Chronic Obstructive Sialadenitis Related to Allergy.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Xuan Zhu,Yan Chen,Deng-Gao Liu,Guang-Yan Yu

Molecular medicine reports 21:2522-2532 PubMed32323817

2020

CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL‑2 and TGF‑β by CD4+ T cells via the STAT5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Wang,Keliang Huang

Journal of ophthalmology 2016:9292346 PubMed27340563

2016

Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiko Sugimoto,Mineo Kondo

Modern pathology : an official journal of the Unit 28:533-44 PubMed25234289

2014

Collagenous gastritis: a morphologic and immunohistochemical study of 40 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Arnason,Ian S Brown,Jeffrey D Goldsmith,William Anderson,Blake H O'Brien,Claire Wilson,Harland Winter,Gregory Y Lauwers

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Eotaxin antibody [EPR5825]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (AB133604)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (AB133604)

Western blot - Anti-Eotaxin antibody [EPR5825] (AB133604)

Western blot - Anti-Eotaxin antibody [EPR5825] (AB133604)

OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (AB133604)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (8)

Product promise