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Rabbit Recombinant Monoclonal Eotaxin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (AB133604), expandable thumbnail
  • Western blot - Anti-Eotaxin antibody [EPR5825] (AB133604), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (AB133604), expandable thumbnail
  • Western blot - Anti-Eotaxin antibody [EPR5825] (AB133604), expandable thumbnail
  • OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (AB133604), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-PWB
Human
Not recommended
Tested
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info
1/1000 - 1/10000
Notes

Note: tested against Eotaxin recombinant protein

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions (PubMed:8597956). Binds to CCR3 (PubMed:8631813).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Eotaxin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR5825

Purification technique

Affinity purification Protein A

Dissociation constant
7.1 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.

Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Associated diseases and disorders

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Eotaxin with Purified ab133604 at 1:100 dilution (1.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604), expandable thumbnail

    Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)

    Blocking and diluting buffer: 5% NFDM/TBST

    Recombinant protein source: Peprotech Inc.
    The region it covers: aa24-aa97(8.3kDa)
    Tag info: N/A

    All lanes: Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/10000 dilution

    All lanes: Human Eotaxin recombinant protein at 0.015 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa

    Observed band size: 8 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Eotaxin with unpurified ab133604 at 1/1000.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604), expandable thumbnail

    Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)

    All lanes: Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/1000 dilution

    All lanes: Eotaxin recombinant protein at 0.01 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 11 kDa

    Observed band size: 8 kDa

  • OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (ab133604), expandable thumbnail

    OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (ab133604)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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