Rabbit Recombinant Monoclonal Eotaxin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18951.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143)")
![Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--western-blot-img54636.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131566.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143)")
![Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--western-blot-img82168.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143)")
![OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--oi-rd-scanning-img83421.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (AB226143)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | WB | |
|---|---|---|---|
| Human | Not recommended | Tested | Expected |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes Note: tested against Eotaxin recombinant protein |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions (PubMed:8597956). Binds to CCR3 (PubMed:8631813).
Alternative names
SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11
Recommended products
Rabbit Recombinant Monoclonal Eotaxin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5825
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 7.1 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab226143 is the carrier-free version of Anti-Eotaxin antibody [EPR5825] ab133604.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.
- Biological function summary
Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.
- Pathways
Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.
- Associated diseases and disorders
Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18951.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
This data was developed using Anti-Eotaxin antibody [EPR5825] ab133604, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Eotaxin with Purified Anti-Eotaxin antibody [EPR5825] ab133604 at 1:100 dilution (1.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
![Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--western-blot-img54636.jpg "Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)")
Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
This data was developed using Anti-Eotaxin antibody [EPR5825] ab133604, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Recombinant protein source: Peprotech Inc. The region it covers: aa24-aa97(8.3kDa). Tag info: N/A.
All lanes: Western blot - Anti-Eotaxin antibody [EPR5825] (Anti-Eotaxin antibody [EPR5825] ab133604) at 1/10000 dilution
All lanes: Human Eotaxin recombinant protein at 0.015 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 8 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131566.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
This data was developed using Anti-Eotaxin antibody [EPR5825] ab133604, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Eotaxin with unpurified Anti-Eotaxin antibody [EPR5825] ab133604 at 1/1000. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--western-blot-img82168.jpg "Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)")
Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
This data was developed using Anti-Eotaxin antibody [EPR5825] ab133604, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Eotaxin antibody [EPR5825] (Anti-Eotaxin antibody [EPR5825] ab133604) at 1/1000 dilution
All lanes: Eotaxin recombinant protein at 0.01 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 8 kDa
![OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143), expandable thumbnail](https://content.abcam.com/products/images/eotaxin-antibody-epr5825-bsa-and-azide-free-ab226143--oi-rd-scanning-img83421.jpg "OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)")
OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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