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AB124162

Anti-Epac1 antibody

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(5 Publications)

Rabbit Polyclonal Epac1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RAPGEF3.

View Alternative Names

CGEF1, EPAC, EPAC1, RAPGEF3, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP, cAMP-regulated guanine nucleotide exchange factor I, EPAC 1, cAMP-GEFI

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Epac1 antibody (AB124162)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Epac1 antibody (AB124162)

Immunofluorescence of EPAC1 in rat brain tissue with ab124162 at 20 ug/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epac1 antibody (AB124162)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epac1 antibody (AB124162)

Immunohistochemical analysis of rat brain tissue, staining Epac1 with ab124162 at 2.5 µg/ml.

Western blot - Anti-Epac1 antibody (AB124162)
  • WB

Unknown

Western blot - Anti-Epac1 antibody (AB124162)

All lanes:

Western blot - Anti-Epac1 antibody (ab124162) at 1 µg/mL

All lanes:

Rat skeletal muscle tissue lysate at 15 µg

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human RAPGEF3. The exact immunogen used to generate this antibody is proprietary information.

O95398

Specificity

At least two isoforms of Epac1 are known to exist; this antibody will detect both isoforms. Epac1 antibody is predicted to not cross-react with Epac2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epac1 also known as Exchange Protein directly Activated by cAMP 1 plays an important role in cellular signaling. It is a guanine nucleotide exchange factor with a molecular mass of about 105 kDa. This protein activates Rap1 and Rap2 small GTPases by promoting the exchange of GDP for GTP. Epac1 is widely expressed in various tissues including the heart brain and immune cells. Its distribution indicates its involvement in multiple physiological processes.
Biological function summary

Epac1 influences cell adhesion proliferation and differentiation. It does not function as a single unit; instead it interacts with other proteins to regulate these cellular processes. Its activity is closely linked with the cAMP signaling pathway where it serves as a mediator of cAMP effects independent of protein kinase A (PKA). The interplay between Epac1 and its associated complexes is essential for proper cellular responses to external stimuli.

Pathways

Epac1 contributes to the cAMP signaling and Ras-related pathways. It functions alongside proteins like Rap1 to relay signals that affect cell adhesion and migration. Within these pathways Epac1 acts as a critical mediator between external signals and intracellular responses. Its interactions with other signaling molecules underline its importance in maintaining cellular functions and responses.

Epac1 has associations with cardiovascular diseases and certain cancers. Its dysregulation can influence heart function linking it with proteins such as PKA and Rap1 in pathological conditions. In cancer altered Epac1 expression may influence tumor growth and metastasis reflecting its involvement with cellular proliferation and migration pathways. Understanding Epac1's role in these conditions can provide insights into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
See full target information RAPGEF3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular vision 28:500-506 PubMed37089701

2022

Epac1 and PKA agonists inhibit ROS to reduce NLRP3 inflammasome proteins in retinal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Youde Jiang,Jena J Steinle

Cell death and differentiation 28:2728-2744 PubMed33934104

2021

PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Xiaoli Sun,Mingjie Wang,Qingmiao Lu,Mengru Gu,Lu Zhou,Qing Hou,Mengzhu Tan,Sudan Wang,Xian Xue,Chunsun Dai

Oncology letters 21:338 PubMed33692870

2021

Dorsal root ganglia NR2B-mediated Epac1-Piezo2 signaling pathway contributes to mechanical allodynia of bone cancer pain.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Ni,Wei Zhang,Yuan Ni,Yan-Ting Mao,Yi Wang,Xiao-Ping Gu,Zheng-Liang Ma

Current biology : CB 29:2339-2350.e5 PubMed31303487

2019

Regulation of Glucose-Dependent Golgi-Derived Microtubules by cAMP/EPAC2 Promotes Secretory Vesicle Biogenesis in Pancreatic β Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn P Trogden,Xiaodong Zhu,Justin S Lee,Christopher V E Wright,Guoqiang Gu,Irina Kaverina

Cell 176:198-212.e15 PubMed30503211

2018

LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Amar N Mirza,Siegen A McKellar,Nicole M Urman,Alexander S Brown,Tyler Hollmig,Sumaira Z Aasi,Anthony E Oro
View all publications
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Product promise

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