AB109415

Anti-Epac1 antibody [EPR1672]

Name: Anti-Epac1 antibody [EPR1672] (ab109415) | Abcam
Brand: Abcam Limited
SKU: AB109415
Price: 635 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(11 Publications)

Rabbit Recombinant Monoclonal Epac1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

CGEF1, EPAC, EPAC1, RAPGEF3, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, Rap1 guanine-nucleotide-exchange factor directly activated by cAMP, cAMP-regulated guanine nucleotide exchange factor I, EPAC 1, cAMP-GEFI

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epac1 antibody [EPR1672] (AB109415)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epac1 antibody [EPR1672] (AB109415)

Immunohistochemical analysis of Epac1 in paraffin-embedded Human kidney tissue using ab109415 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Epac1 antibody [EPR1672] (AB109415)

All lanes:

Western blot - Anti-Epac1 antibody [EPR1672] (ab109415) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human heart lysate at 10 µg

Lane 4:

HUVEC cell lysate at 10 µg

Predicted band size: 104 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Epac1 antibody [EPR1672]
Carrier free

Anti-Epac1 antibody [EPR1672] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1672

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval is recommended.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epac1 also known as Exchange Protein directly Activated by cAMP 1 plays an important role in cellular signaling. It is a guanine nucleotide exchange factor with a molecular mass of about 105 kDa. This protein activates Rap1 and Rap2 small GTPases by promoting the exchange of GDP for GTP. Epac1 is widely expressed in various tissues including the heart brain and immune cells. Its distribution indicates its involvement in multiple physiological processes.

Biological function summary

Epac1 influences cell adhesion proliferation and differentiation. It does not function as a single unit; instead it interacts with other proteins to regulate these cellular processes. Its activity is closely linked with the cAMP signaling pathway where it serves as a mediator of cAMP effects independent of protein kinase A (PKA). The interplay between Epac1 and its associated complexes is essential for proper cellular responses to external stimuli.

Pathways

Epac1 contributes to the cAMP signaling and Ras-related pathways. It functions alongside proteins like Rap1 to relay signals that affect cell adhesion and migration. Within these pathways Epac1 acts as a critical mediator between external signals and intracellular responses. Its interactions with other signaling molecules underline its importance in maintaining cellular functions and responses.

Epac1 has associations with cardiovascular diseases and certain cancers. Its dysregulation can influence heart function linking it with proteins such as PKA and Rap1 in pathological conditions. In cancer altered Epac1 expression may influence tumor growth and metastasis reflecting its involvement with cellular proliferation and migration pathways. Understanding Epac1's role in these conditions can provide insights into potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.

See full target information RAPGEF3

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:9043 PubMed40091082

2025

Plakoglobin does not participate in endothelial barrier stabilization mediated by cAMP.

Applications

Unspecified application

Species

Unspecified reactive species

Ibrahim Hamad,Sara Sepic,Sina Moztarzadeh,Alexander García-Ponce,Jens Waschke,Mariya Y Radeva

The Journal of biological chemistry 300:105509 PubMed38042493

2023

N-acetyl cysteine turns EPAC activators into potent killers of acute lymphoblastic leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Richartz,Wojciech Pietka,Ajay Yadav,Monica Bostad,Sampada Bhagwat,Soheil Naderi,Elin Hallan Naderi,Trond Stokke,Ellen Ruud,Heidi Kiil Blomhoff

Frontiers in endocrinology 13:918652 PubMed35865309

2022

Electroacupuncture Attenuates Post-Inflammatory IBS-Associated Visceral and Somatic Hypersensitivity and Correlates With the Regulatory Mechanism of Epac1-Piezo2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Guo,Lu Chen,Yu-Hang Wang,Ya-Fang Song,Zhan-Hao Zhao,Ting-Ting Zhao,Zhi-Ying Lin,Dong-Mei Gu,Yun-Qi Liu,Yong-Jun Peng,Li-Xia Pei,Jian-Hua Sun

Translational research : the journal of laboratory and clinical medicine 244:101-113 PubMed35114419

2022

In Vivo Cardiac-specific Expression of Adenylyl Cyclase 4 Gene Protects against Klotho Deficiency-induced Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Chen,Shirley Wang,Zhongjie Sun

Frontiers in pharmacology 12:722040 PubMed34819854

2021

Arecoline Enhances Phosphodiesterase 4A Activity to Promote Transforming Growth Factor-β-Induced Buccal Mucosal Fibroblast Activation via cAMP-Epac1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Lihua Gao,Chunsheng Shao,Mingsi Deng,Liangjian Chen

Acta pharmacologica Sinica 43:1349-1359 PubMed34697419

2021

Iptakalim improves cerebral microcirculation in mice after ischemic stroke by inhibiting pericyte contraction.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Bing Guo,Yin-Feng Dong,Zhi Yin,Zhen-Yu Cai,Jin Yang,Juan Ji,Yu-Qin Sun,Xin-Xin Huang,Teng-Fei Xue,Hong Cheng,Xi-Qiao Zhou,Xiu-Lan Sun

Cells 10: PubMed34571955

2021

Identification and Characterization of an Affimer Affinity Reagent for the Detection of the cAMP Sensor, EPAC1.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna K Buist,Urszula Luchowska-Stańska,Boy van Basten,Jessica Valli,Brian O Smith,George S Baillie,Colin Rickman,Bryon Ricketts,Alex Davidson,Ryan Hannam,Joanne Sunderland,Stephen J Yarwood

European journal of pain (London, England) 25:2039-2049 PubMed34101933

2021

The role of G-protein-coupled receptor kinase 2 in diabetic mechanical hyperalgesia in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Hua Xu,Rui-Qin Du,Lin Li,Lin-Lin Yang,Yi Zhang,Quan-Min Li

Cells 9: PubMed32992982

2020

Epac1 Is Crucial for Maintenance of Endothelial Barrier Function through A Mechanism Partly Independent of Rac1.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander García-Ponce,Katharina Schuster,Stein-Ove Døskeland,Rolf K Reed,Fitz-Roy E Curry,Jens Waschke,Mariya Y Radeva

Molecular vision 23:1-7 PubMed28210097

2017

Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Youde Jiang,Adam Chahine,Elizabeth Curtiss,Jena J Steinle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Epac1 antibody [EPR1672]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epac1 antibody [EPR1672] (AB109415)

Western blot - Anti-Epac1 antibody [EPR1672] (AB109415)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise