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AB193665

Anti-Epac2 antibody [EPR15246]

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(4 Publications)

Rabbit Recombinant Monoclonal Epac2 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

CGEF2, EPAC2, RAPGEF4, Rap guanine nucleotide exchange factor 4, Exchange factor directly activated by cAMP 2, Exchange protein directly activated by cAMP 2, cAMP-regulated guanine nucleotide exchange factor II, EPAC 2, cAMP-GEFII

1 Images
Western blot - Anti-Epac2 antibody [EPR15246] (AB193665)
  • WB

Supplier Data

Western blot - Anti-Epac2 antibody [EPR15246] (AB193665)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Epac2 antibody [EPR15246] (ab193665) at 1/5000 dilution

Lane 1:

Rat brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

Observed band size: 116 kDa

false

  • Carrier free

    Anti-Epac2 antibody [EPR15246] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15246

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epac2 also known as exchange protein directly activated by cAMP 2 functions as a guanine nucleotide exchange factor (GEF) for the small GTPases Rap1 and Rap2. Epac2 weighs approximately 110 kDa and is expressed in multiple tissues with high expression in the brain and pancreas. It responds to the second messenger cAMP acting as a critical link between G protein-coupled receptors (GPCRs) and downstream signal transduction pathways. Epac2 has two important domains: the regulatory cAMP-binding domain and the catalytic CDC25 homology domain allowing it to operate as a multi-functional signaling node.
Biological function summary

Epac2 modulates intracellular signaling processes by mediating the activation of small GTPases. This activation influences cell adhesion cell junction formation and insulin secretion. Epac2 does not function in isolation; it forms a complex with other signaling molecules impacting various cellular processes. In the brain Epac2 also plays a significant role in synaptic plasticity and memory formation integrating signals that modulate neuronal activity.

Pathways

Epac2 integrates into key signaling cascades such as the cAMP signaling pathway and the MAPK/ERK pathway. It participates in the cAMP signaling pathway by facilitating conversion of cAMP to active GTP-bound Rap proteins. In doing so Epac2 interacts closely with proteins like protein kinase A (PKA) and Rap1. Each component plays a distinct role in transducing extracellular signals into cellular responses promoting a coordinated cellular activity profile.

Research connects Epac2 to conditions such as type 2 diabetes and autism spectrum disorders (ASD). In type 2 diabetes Epac2 influences insulin secretion and its dysregulation may be linked to impaired glucose homeostasis. In ASD altered Epac2 signaling impacts neuronal communication and plasticity. Beyond this Epac2's interaction with other regulatory proteins like Rap1 plays a role in these disease contexts by affecting cellular functions critical for metabolic and neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).
See full target information RAPGEF4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Neuropathology and applied neurobiology 47:625-639 PubMed33345400

2021

RAPGEF2 mediates oligomeric Aβ-induced synaptic loss and cognitive dysfunction in the 3xTg-AD mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

You-Na Jang,HoChung Jang,Gyu Hyun Kim,Jeong-Eun Noh,Keun-A Chang,Kea Joo Lee

Oncology letters 17:5080-5086 PubMed31186720

2019

Overexpression of EPAC2 reduces the invasion of glioma cells via MMP-2.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Jiang,Yan Zhuang,Wang-Cun Zu,Lei Jiao,Seidu A Richard,Shiming Zhang

Progress in neuro-psychopharmacology & biological psychiatry 90:62-75 PubMed30391306

2018

FCPR16, a novel phosphodiesterase 4 inhibitor, produces an antidepressant-like effect in mice exposed to chronic unpredictable mild stress.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuping Zhong,Hui Yu,Chang Huang,Jiahong Zhong,Haitao Wang,Jiangping Xu,Yufang Cheng

Molecular vision 23:1-7 PubMed28210097

2017

Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Youde Jiang,Adam Chahine,Elizabeth Curtiss,Jena J Steinle
View all publications
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Product promise

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