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AB251174

Anti-Epac2 antibody [EPR15246] - BSA and Azide free

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Rabbit Recombinant Monoclonal Epac2 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse samples.

View Alternative Names

CGEF2, EPAC2, RAPGEF4, Rap guanine nucleotide exchange factor 4, Exchange factor directly activated by cAMP 2, Exchange protein directly activated by cAMP 2, cAMP-regulated guanine nucleotide exchange factor II, EPAC 2, cAMP-GEFII

1 Images
Western blot - Anti-Epac2 antibody [EPR15246] - BSA and Azide free (AB251174)
  • WB

Supplier Data

Western blot - Anti-Epac2 antibody [EPR15246] - BSA and Azide free (AB251174)

This data was developed using ab193665, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Epac2 antibody [EPR15246] (<a href='/en-us/products/primary-antibodies/epac2-antibody-epr15246-ab193665'>ab193665</a>) at 1/5000 dilution

Lane 1:

Rat brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

Observed band size: 116 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15246

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251174 is the carrier-free version of ab193665.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epac2 also known as exchange protein directly activated by cAMP 2 functions as a guanine nucleotide exchange factor (GEF) for the small GTPases Rap1 and Rap2. Epac2 weighs approximately 110 kDa and is expressed in multiple tissues with high expression in the brain and pancreas. It responds to the second messenger cAMP acting as a critical link between G protein-coupled receptors (GPCRs) and downstream signal transduction pathways. Epac2 has two important domains: the regulatory cAMP-binding domain and the catalytic CDC25 homology domain allowing it to operate as a multi-functional signaling node.
Biological function summary

Epac2 modulates intracellular signaling processes by mediating the activation of small GTPases. This activation influences cell adhesion cell junction formation and insulin secretion. Epac2 does not function in isolation; it forms a complex with other signaling molecules impacting various cellular processes. In the brain Epac2 also plays a significant role in synaptic plasticity and memory formation integrating signals that modulate neuronal activity.

Pathways

Epac2 integrates into key signaling cascades such as the cAMP signaling pathway and the MAPK/ERK pathway. It participates in the cAMP signaling pathway by facilitating conversion of cAMP to active GTP-bound Rap proteins. In doing so Epac2 interacts closely with proteins like protein kinase A (PKA) and Rap1. Each component plays a distinct role in transducing extracellular signals into cellular responses promoting a coordinated cellular activity profile.

Research connects Epac2 to conditions such as type 2 diabetes and autism spectrum disorders (ASD). In type 2 diabetes Epac2 influences insulin secretion and its dysregulation may be linked to impaired glucose homeostasis. In ASD altered Epac2 signaling impacts neuronal communication and plasticity. Beyond this Epac2's interaction with other regulatory proteins like Rap1 plays a role in these disease contexts by affecting cellular functions critical for metabolic and neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).
See full target information RAPGEF4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com