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AB185704

Anti-EPB41 antibody

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(2 Publications)

Rabbit Polyclonal EPB41 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EPB41 aa 200-500.

View Alternative Names

E41P, EPB41, Protein 4.1, P4.1, 4.1R, Band 4.1, EPB4.1, Erythrocyte membrane protein band 4.1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-EPB41 antibody (AB185704)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPB41 antibody (AB185704)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185704. Blue DAPI for nuclear staining.

Western blot - Anti-EPB41 antibody (AB185704)
  • WB

Supplier Data

Western blot - Anti-EPB41 antibody (AB185704)

All lanes:

Western blot - Anti-EPB41 antibody (ab185704) at 1/500 dilution

Lane 1:

293T cell lysate

Lane 2:

mouse brain lysate

Lane 3:

mouse liver lysate

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EPB41 aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

P11171

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Primary Antibodies

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Anti-Ankyrin erythroid/ANK antibody [S388A-10]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein 4.1 also known as EPB41 plays an important role in maintaining the mechanical stability and elasticity of the erythrocyte membrane. This protein exists as a component of the erythrocyte membrane skeleton where it interacts with spectrin and actin contributing to the cytoskeletal network. It weighs approximately 80kDa. EPB41 expression is not limited to erythrocytes; it's found in various tissues including the brain and kidney which indicates its diverse functional roles.
Biological function summary

The protein supports cell shape and mechanical properties by linking the cytoskeleton to the plasma membrane. EPB41 is involved in the membrane-cytoskeletal junctional complex where it interacts with other proteins such as spectrin and glycophorin C. This interaction is key in erythrocytes to prevent cell lysis under mechanical stress. Beyond its role in erythrocytes EPB41 assists in protein stabilization and localization within the plasma membrane in other cell types.

Pathways

EPB41 plays an important part in the erythrocyte cell surface structure pathway as well as cell signaling pathways in non-erythroid cells. In the former EPB41 works closely with spectrin and actin to maintain the cellular integrity necessary for oxygen transport. Furthermore in cell signaling pathways its interaction with ankyrin influences membrane organization and signal transduction which are vital for diverse cellular functions.

Mutations or malfunctions in EPB41 correlate with conditions such as hereditary elliptocytosis and neurodegenerative disorders. Hereditary elliptocytosis results from defects in erythrocyte membrane proteins where EPB41 interplays with spectrin to contribute to the disorder. In neurodegenerative diseases alterations in EPB41 may affect brain tissue stability and function due to its role in sustaining membrane mechanics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase (PubMed : 23870127).
See full target information EPB41
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1524930 PubMed40207224

2025

Exploring the possible mechanism of low-dose naloxone exposure improving the immune microenvironment of gastric cancer tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangzhen Min,Yan Ma,Mingyue Lv,Xiaoxi Li,Renjun Lv,Xiaoyong Zhao,Yufang Leng

Nature communications 12:1172 PubMed33608523

2021

20S proteasomes secreted by the malaria parasite promote its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-Rudzki
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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