AB244499

Anti-EPB41L1 antibody

Name: Anti-EPB41L1 antibody (ab244499) | Abcam
Brand: Abcam Limited
SKU: AB244499
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal EPB41L1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EPB41L1 aa 600-750.

View Alternative Names

KIAA0338, EPB41L1, Band 4.1-like protein 1, Erythrocyte membrane protein band 4.1-like 1, Neuronal protein 4.1, 4.1N

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Paraffin-embedded human skeletal muscle tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis. Low expression, as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Paraffin-embedded human colon tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Paraffin-embedded human adrenal gland tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Paraffin-embedded human cerebral cortex tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-EPB41L1 antibody (AB244499)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPB41L1 antibody (AB244499)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for EPB41L1 (green) using ab244499 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Paraffin-embedded human kidney tissue stained for EPB41L1 using ab244499 at 1/200 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-EPB41L1 antibody (AB244499)

All lanes:

Western blot - Anti-EPB41L1 antibody (ab244499) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EPB41L1 aa 600-750. The exact immunogen used to generate this antibody is proprietary information.

Q9H4G0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EPB41L1 gene encodes for protein 4.1N often referred to as EPB41L1 which is a member of the protein 4.1 superfamily. This protein has a molecular mass of approximately 145 kDa. EPB41L1 is expressed mostly in the nervous system including various brain regions and peripheral nerves. This protein is pivotal for maintaining structural integrity and stability of the cytoskeleton. EPB41L1 interacts with spectrin-actin complexes contributing to cellular shape and mechanical properties.

Biological function summary

Protein 4.1N plays a role in cellular processes like cell adhesion and migration. It participates in the formation of multiprotein complexes that include cytoskeletal elements and membrane proteins. This protein also influences neuronal function through its interaction with ion channels and synaptic proteins. EPB41L1 is integral to neuronal adaptability and contributes to synaptic plasticity processes impacting neuron communication.

Pathways

EPB41L1 is heavily involved in the signal transduction pathways within neurons. This protein associates with the Ras signaling pathway and can engage with proteins like Ras-related proteins that influence cell proliferation and differentiation. Additionally EPB41L1 is part of the integrin signaling pathway where it modulates cellular interactions with the extracellular matrix and impacts cell movement.

EPB41L1 has connections to neurodevelopmental and psychiatric disorders including schizophrenia and autism spectrum disorders. Impaired EPB41L1 function may disrupt neuronal communication leading to cognitive and behavioral symptoms. The protein is also linked to interactions with DISC1 a protein associated with psychiatric conditions providing insights into its role in the etiology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases.

See full target information EPB41L1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed37580082

2023

Neuroprotective effects of hepatoma-derived growth factor in models of Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin Voelkl,Sara Gutiérrez-Ángel,Sophie Keeling,Seda Koyuncu,Miguel da Silva Padilha,Dennis Feigenbutz,Thomas Arzberger,David Vilchez,Rüdiger Klein,Irina Dudanova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPB41L1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Immunocytochemistry/ Immunofluorescence - Anti-EPB41L1 antibody (AB244499)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L1 antibody (AB244499)

Western blot - Anti-EPB41L1 antibody (AB244499)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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