AB250096

Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal EPB41L1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

KIAA0338, EPB41L1, Band 4.1-like protein 1, Erythrocyte membrane protein band 4.1-like 1, Neuronal protein 4.1, 4.1N

3 Images

Flow Cytometry (Intracellular) - Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free (AB250096)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free (AB250096)

This data was developed using ab179453, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Neuro 2a cells labeling EPB41L1 with ab179453 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

Supplier Data

Western blot - Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free (AB250096)

This data was developed using ab179453, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EPB41L1 antibody [EPR11090] (<a href='/en-us/products/primary-antibodies/epb41l1-antibody-epr11090-ab179453'>ab179453</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Neuro 2a lysate at 10 µg

Predicted band size: 99 kDa

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Supplier Data

Immunoprecipitation - Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free (AB250096)

This data was developed using ab179453, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab179453 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-EPB41L1 antibody [EPR11090] (<a href='/en-us/products/primary-antibodies/epb41l1-antibody-epr11090-ab179453'>ab179453</a>)

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11090

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250096 is the carrier-free version of ab179453.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EPB41L1 gene encodes for protein 4.1N often referred to as EPB41L1 which is a member of the protein 4.1 superfamily. This protein has a molecular mass of approximately 145 kDa. EPB41L1 is expressed mostly in the nervous system including various brain regions and peripheral nerves. This protein is pivotal for maintaining structural integrity and stability of the cytoskeleton. EPB41L1 interacts with spectrin-actin complexes contributing to cellular shape and mechanical properties.

Biological function summary

Protein 4.1N plays a role in cellular processes like cell adhesion and migration. It participates in the formation of multiprotein complexes that include cytoskeletal elements and membrane proteins. This protein also influences neuronal function through its interaction with ion channels and synaptic proteins. EPB41L1 is integral to neuronal adaptability and contributes to synaptic plasticity processes impacting neuron communication.

Pathways

EPB41L1 is heavily involved in the signal transduction pathways within neurons. This protein associates with the Ras signaling pathway and can engage with proteins like Ras-related proteins that influence cell proliferation and differentiation. Additionally EPB41L1 is part of the integrin signaling pathway where it modulates cellular interactions with the extracellular matrix and impacts cell movement.

EPB41L1 has connections to neurodevelopmental and psychiatric disorders including schizophrenia and autism spectrum disorders. Impaired EPB41L1 function may disrupt neuronal communication leading to cognitive and behavioral symptoms. The protein is also linked to interactions with DISC1 a protein associated with psychiatric conditions providing insights into its role in the etiology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function to confer stability and plasticity to neuronal membrane via multiple interactions, including the spectrin-actin-based cytoskeleton, integral membrane channels and membrane-associated guanylate kinases.

See full target information EPB41L1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:74 PubMed40069860

2025

Inhibition of histone deacetylase 6 alleviates neuropathic pain via direct regulating post-translation of spinal STAT3 and decreasing downstream C-C Motif Chemokine Ligand 7 synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhexi Chi,Bo Lu,Rongjun Liu,Chen Pan,Bo Meng,Xiuzhong Xing,Hui Yuan,Xuewei Wu,Yushan Chen,Yuxuan Ren,Wenwei Wu,Mengmeng Miao,Junping Chen,Xiaowei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free (AB250096)

Western blot - Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free (AB250096)

Immunoprecipitation - Anti-EPB41L1 antibody [EPR11090] - BSA and Azide free (AB250096)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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