AB99375

Anti-EPB41L2 antibody

Name: Anti-EPB41L2 antibody (ab99375) | Abcam
Brand: Abcam Limited
SKU: AB99375
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal EPB41L2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EPB41L2 aa 100-150.

View Alternative Names

Band 4.1-like protein 2, Erythrocyte membrane protein band 4.1-like 2, Generally expressed protein 4.1, 4.1G, EPB41L2

2 Images

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Immunoprecipitation - Anti-EPB41L2 antibody (AB99375)

B>Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99375 at 3 μg/mg lysate; 20% of immunoprecipitate loaded in lane

Western Blot : EPB41L2 antibody (ab99375) at 1 μg/ml

developed using the ECL technique

Exposure time : 10 seconds

Predicted band size : 113 kDa

All lanes:

Immunoprecipitation - Anti-EPB41L2 antibody (ab99375)

Predicted band size: 113 kDa

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Western blot - Anti-EPB41L2 antibody (AB99375)

All lanes:

Western blot - Anti-EPB41L2 antibody (ab99375) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 113 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EPB41L2 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

O43491

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPB41L2 also known as erythrocyte membrane protein band 4.1-like 2 has a mechanical role in stabilizing cell membranes by linking the cytoskeleton to the plasma membrane. This protein weighs approximately 90 kDa. EPB41L2 is widely expressed in various tissues with a notable presence in the brain and nervous system where it impacts neuronal structure and signal transmission. The structural integrity that EPB41L2 provides to cellular membranes aids in maintaining cellular shape and signal transduction.

Biological function summary

EPB41L2 is a critical component of the 4.1 protein family which regulates cytoskeletal architecture and cellular adhesion. This protein interacts with spectrin and actin playing a role in the formation and stability of the spectrin-actin network. Within cells EPB41L2 associates with lipid rafts which are small dynamic cholesterol-enriched domains in the membrane facilitating signal transduction and protein sorting. This positioning suggests its involvement in complex cellular processes requiring membrane organizing capabilities.

Pathways

EPB41L2 plays essential roles in the pathways regulating cytoskeleton rearrangement and cell adhesion. It is particularly implicated in the Rho GTPase signaling pathway where it interacts with proteins such as RhoA influencing cytoskeletal dynamics and cell motility. Additionally EPB41L2 is part of the regulation pathway of cell shape and polarity particularly in neurons affecting interactions with proteins involved in axonal guidance and synaptic function.

EPB41L2 has associations with conditions affecting the nervous system such as neurodevelopmental disorders and certain types of epilepsy. EPB41L2's dysregulation may contribute to altered neuronal connectivity and signal transmission exacerbating these conditions. Additionally EPB41L2 interactions with ankyrin or neurofascin could be disturbed in pathological states leading to instability in neural structures and potentially contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase (PubMed : 23870127).

See full target information EPB41L2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:820 PubMed30778055

2019

Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Angela Gomes de Castro,Hanna Wildhagen,Shama Sograte-Idrissi,Christoffer Hitzing,Mascha Binder,Martin Trepel,Niklas Engels,Felipe Opazo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPB41L2 antibody

Immunoprecipitation - Anti-EPB41L2 antibody (AB99375)

Western blot - Anti-EPB41L2 antibody (AB99375)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise