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AB112043

Anti-EPC1 antibody

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(1 Publication)

Rabbit Polyclonal EPC1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EPC1 aa 300-400.

View Alternative Names

Enhancer of polycomb homolog 1, EPC1

2 Images
Immunoprecipitation - Anti-EPC1 antibody (AB112043)
  • IP

Unknown

Immunoprecipitation - Anti-EPC1 antibody (AB112043)

Detection of EPC1 by Western Blot of Immunprecipitate.
ab112043 at 1µg/ml staining EPC1 in HeLa whole cell lysate immunoprecipitated using ab112043 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-EPC1 antibody (ab112043)

Predicted band size: 93 kDa

false

Western blot - Anti-EPC1 antibody (AB112043)
  • WB

Unknown

Western blot - Anti-EPC1 antibody (AB112043)

All lanes:

Western blot - Anti-EPC1 antibody (ab112043) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 93 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EPC1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q9H2F5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPC1 also called Enhancer of Polycomb homolog 1 is a nuclear protein with a mass of approximately 89 kDa. It plays a role in chromatin remodeling as part of the NuA4 histone acetyltransferase complex. EPC1 is expressed in various tissues with notable expression in embryonic tissues and somatic cells undergoing differentiation. There is significant involvement of EPC1 in transcriptional regulation through modification of chromatin structure.
Biological function summary

As a component of the NuA4 complex EPC1 contributes to the acetylation of histones impacting gene expression. This complex is involved in chromatin modification and facilitates transcriptional activation of genes related to cell cycle progression DNA repair and development. By influencing these vital processes EPC1 affects cell proliferation and differentiation highlighting its role in maintaining genomic stability and cellular integrity.

Pathways

EPC1 engages in key signaling pathways such as the Notch signaling pathway and the DNA damage response pathway. These pathways are critical in regulating cell fate decisions and maintaining genomic stability. EPC1 interacts with proteins like EP400 which is part of the same complex as well as other transcription factors and co-factors that mediate its effects on gene expression and chromatin dynamics.

EPC1 shows association with certain cancers particularly those with dysregulated chromatin structures and developmental disorders. Aberrant function or expression of EPC1 may contribute to tumorigenesis especially in hematological malignancies. Mutations or dysregulation in associated proteins like EP400 and others within the NuA4 complex can further impact disease progression implicating EPC1 as a potential target for therapeutic intervention in these contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the NuA4 histone acetyltransferase (HAT) complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed : 14966270). The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR) (PubMed : 27153538). The NuA4 complex is also required for spermatid development by promoting acetylation of histones : histone acetylation is required for histone replacement during the transition from round to elongating spermatids (By similarity). In the NuA4 complex, EPC1 is required to recruit MBTD1 into the complex (PubMed : 32209463).
See full target information EPC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 44:117-33 PubMed26350215

2015

Epigenetic factor EPC1 is a master regulator of DNA damage response by interacting with E2F1 to silence death and activate metastasis-related gene signatures.

Applications

ChIP

Species

Unspecified reactive species

Yajie Wang,Vijay Alla,Deborah Goody,Shailendra K Gupta,Alf Spitschak,Olaf Wolkenhauer,Brigitte M Pützer,David Engelmann
View all publications
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Product promise

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