AB231223

Anti-EpCAM antibody

Name: Anti-EpCAM antibody (ab231223) | Abcam
Brand: Abcam Limited
SKU: AB231223

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal EPCAM antibody. Suitable for WB and reacts with Chicken samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Chicken EPCAM aa 1-300.

View Alternative Names

TACSTD1, RCJMB04_29h4, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Tumor-associated calcium signal transducer 1

3 Images

Supplier Data

Western blot - Anti-EpCAM antibody (AB231223)

All lanes:

Western blot - Anti-EpCAM antibody (ab231223) at 3 µg/mL

All lanes:

Chicken kidney lysate

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-EpCAM antibody (AB231223)

All lanes:

Western blot - Anti-EpCAM antibody (ab231223) at 2 µg/mL

All lanes:

Recombinant chicken EpCAM protein

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-EpCAM antibody (AB231223)

All lanes:

Western blot - Anti-EpCAM antibody (ab231223) at 3 µg/mL

All lanes:

Chicken stomach lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Chicken

Applications

applications

Immunogen

Recombinant Fragment Protein within Chicken EPCAM aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q5F381

style

left-column

Alternative Products

Primary Antibodies

AB213500

Anti-EpCAM antibody [EPR20532-222]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20532-222] (AB213500)

Primary Antibodies

AB124825

Anti-EpCAM antibody [EPR677(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Primary Antibodies

AB225894

Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free (AB225894)

Primary Antibodies

AB221552

Anti-EpCAM antibody [EPR20533-63]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] (AB221552)

Primary Antibodies

AB213501

Anti-EpCAM antibody [EPR20533-266]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] (AB213501)

Primary Antibodies

AB32392

Anti-EpCAM antibody [E144]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-EpCAM antibody [E144] (AB32392)

Primary Antibodies

AB223582

Anti-EpCAM antibody [EPR20532-225]

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] (AB223582)

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chicken": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

(Microbial infection) Facilitates Eimeria tenella adhesion and invasion.

See full target information EPCAM

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic translation 33:90-106 PubMed35330941

2022

3D printed porous sulfonated polyetheretherketone scaffold for cartilage repair: Potential and limitation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguo Yuan,Teng Long,Jue Zhang,Zhuocheng Lyu,Wei Zhang,Xiangchao Meng,Jin Qi,You Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com