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AB7504

Anti-EpCAM antibody [Ber-EP4]

5

(4 Reviews)

|

(31 Publications)

Mouse Monoclonal EPCAM antibody. Suitable for IHC-P, IHC-FoFr, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 31 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Ber-EP4] (AB7504)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Ber-EP4] (AB7504)

ab7504 staining EpCAM in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. Staining was detected using DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Ber-EP4] (AB7504)
  • IHC-P

AbReview19877****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Ber-EP4] (AB7504)

ab7504 staining EpCAM in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 1 hour at 20°C; antigen retrieval was by heat mediation (microwave) in a commercial retrieval buffer. Samples were incubated with primary antibody (1/250 in 1% serum) for 12 hours at 4°C. A Biotin-conjugated Horse anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Eva Schmelzer

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Ber-EP4

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-FoFr, ICC/IF, IHC-Fr, IHC-P

applications

Immunogen

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

Specificity

Epithelial specific antigen is cell surface glycoprotein and is broadly distributed in epithelial cells and displays a highly conserved expression in carcinomas. Epithelial specific antigen has been know to play an important role as tumor-cell marker in lymph nodes from patients with esophageal carcinoma. Epithelial specific antigen can be used to distinguish between basal cell and basosquamous carcinomas and squamous cell carcinoma of the skin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
See full target information EPCAM
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Cell regeneration (London, England) 13:17 PubMed39269631

2024

Endogenous retroviral ERVH48-1 promotes human urine cell reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yuling Peng,Jieying Zhu,Qi Zhang,Ran Zhang,Zhenhua Wang,Zesen Ye,Ning Ma,Dajiang Qin,Duanqing Pei,Dongwei Li

Cell reports. Medicine 5:101692 PubMed39163864

2024

CTC-derived pancreatic cancer models serve as research tools and are suitable for precision medicine approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Tang,Quan Zheng,Qi Wang,Yaru Zhao,Preeta Ananthanarayanan,Chiara Reina,Berina Šabanović,Ke Jiang,Ming-Hsin Yang,Clara Csilla Meny,Huimin Wang,Mette Ø Agerbaek,Thomas Mandel Clausen,Tobias Gustavsson,Chenlei Wen,Felice Borghi,Alfredo Mellano,Elisabetta Fenocchio,Vanesa Gregorc,Anna Sapino,Thor G Theander,Da Fu,Alexandra Aicher,Ali Salanti,Baiyong Shen,Christopher Heeschen

Genome medicine 16:98 PubMed39138551

2024

Spatial multiomics reveals a subpopulation of fibroblasts associated with cancer stemness in human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Yu Jing,Dan Liu,Na Feng,Hui Dong,He-Qi Wang,Xi Yan,Xu-Feng Chen,Min-Cheng Qu,Ping Lin,Bin Yi,Feiling Feng,Lei Chen,Hong-Yang Wang,Hong Li,Yu-Fei He

Scientific reports 14:10199 PubMed38702437

2024

Clinical impact of high-quality testing for peritoneal lavage cytology in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Tanemura,Kenta Furukawa,Manabu Mikamori,Tadafumi Asaoka,Hironao Yasuoka,Daiki Marukawa,Yasuo Urata,Daisaku Yamada,Shogo Kobayashi,Hidetoshi Eguchi

Med (New York, N.Y.) 4:554-579.e9 PubMed37572651

2023

Organoid co-culture model of the human endometrium in a fully synthetic extracellular matrix enables the study of epithelial-stromal crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Juan S Gnecco,Alexander Brown,Kira Buttrey,Clara Ives,Brittany A Goods,Lauren Baugh,Victor Hernandez-Gordillo,Megan Loring,Keith B Isaacson,Linda G Griffith

Methods in molecular biology (Clifton, N.J.) 2668:257-276 PubMed37140802

2023

Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Bernuz,Arnau Pallarès-Rusiñol,Rosanna Rossi,Carolina Fernández-Senac,Mercè Martí,María Isabel Pividori

The Prostate 83:277-285 PubMed36372998

2022

Presence of cells in the polyaneuploid cancer cell (PACC) state predicts the risk of recurrence in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Levent Trabzonlu,Kenneth J Pienta,Bruce J Trock,Angelo M De Marzo,Sarah R Amend

Journal of clinical medicine 11: PubMed35456276

2022

Phase I Targeted Combination Trial of Sorafenib and GW5074 in Patients with Advanced Refractory Solid Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Chang Kao,Ching-Liang Ho,Ming-Hsin Yang,Yi-Ta Tsai,Shu-Yu Liu,Ping-Ying Chang,Yi-Ying Wu,Jia-Hong Chen,Tzu-Chuan Huang,Ren-Hua Yehn,Ming-Shen Dai,Yeu-Chin Chen,Guang-Huan Sun,Tai-Lung Cha

Frontiers in cell and developmental biology 10:810687 PubMed35223840

2022

Spatial Multiomics Analysis Reveals Only Minor Genetic and Epigenetic Changes in Human Liver Cancer Stem-Like Cells Compared With Other Tumor Parenchymal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Liu,Hong Li,Hui Dong,Mincheng Qu,Liguang Yang,Lina Chen,Yixue Li,Hongyang Wang,Yufei He

iScience 24:103478 PubMed34841222

2021

Pannexin-1 channel opening is critical for COVID-19 pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ross Luu,Silvana Valdebenito,Eliana Scemes,Antonio Cibelli,David C Spray,Maximiliano Rovegno,Juan Tichauer,Andrea Cottignies-Calamarte,Arielle Rosenberg,Calude Capron,Sandrine Belouzard,Jean Dubuisson,Djillali Annane,Geoffroy Lorin de la Grandmaison,Elisabeth Cramer-Bordé,Morgane Bomsel,Eliseo Eugenin
View all publications
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Product promise

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